| 两个甘蓝自交系自交不亲和S单元型的初步鉴定 |
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| 引用本文： | 田磊,庄木,刘玉梅,杨丽梅,张扬勇,方智远. 两个甘蓝自交系自交不亲和S单元型的初步鉴定[J]. 中国蔬菜, 2011, 1(4): 8-12 |
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| 作者姓名： | 田磊 庄木 刘玉梅 杨丽梅 张扬勇 方智远 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所，北京 100081 |
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| 基金项目： | 农业部大宗蔬菜产业技术体系项目(hycytx-35),农业部园艺作物遗传改良重点开放实验室项目; 中央级公益性科研院所基本科研业务费专项(201011) |
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| 摘 要： | 根据甘蓝SRK基因序列保守区设计特异性引物，通过PCR扩增和克隆测序，结合生物信息学分析方法对两个甘蓝自交系材料的S单元型进行初步鉴定。BLAST分析表明：甘蓝自交系96-100与Brassica oleracea的SRK29基因核苷酸序列相似性最高（91 %），但蛋白序列与S14相似性最高（在84 %的序列覆盖度下相似性达100 %）。自交系俄引165与Brassica oleracea的BoSRK28基因核苷酸序列相似性达100 %，推测其S单元型为S28。
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| 关 键 词： | 甘蓝 自交不亲和性 S单元型 SRK基因 |
| 收稿时间： | 2010-12-15; |
Preliminary Identification of S Haplotypes of Two Self-incompatible Lines in Cabbage (Brassica oleracea var. capitata L.) |
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| TIAN Lei,ZHUANG Mu,LIU Yu-mei,YANG Li-mei,ZHANG Yang-yong,FANG Zhi-yuan. Preliminary Identification of S Haplotypes of Two Self-incompatible Lines in Cabbage (Brassica oleracea var. capitata L.)[J]. China Vegetables, 2011, 1(4): 8-12 |
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| Authors: | TIAN Lei ZHUANG Mu LIU Yu-mei YANG Li-mei ZHANG Yang-yong FANG Zhi-yuan |
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| Affiliation: | Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | According to the conserved sequence of S locus kinase(SRK)gene in cabbage(Brassica oleracea var.capitata L.),a pair of SRK-specific primers was designed.The S haplotypes of 2 inbred lines in cabbage were identified by PCR amplification and DNA sequencing,combined with bio-informatics analysis.BLAST results indicated that the self-incompatible line 96-100 showed 91 % similarity in nucleotide sequence with SRK29 gene in cabbage,and 100 % similarity under 84 % query coverage of protein residue with S14 haploty... |
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| Keywords: | Cabbage Self-incompatibility S haplotype SRK gene |
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