环境因子对BB·L/W大鼠青少年型糖尿病的影响:Ⅰ.无菌环境的作用

本实验完成了无菌劳邦迪BB/W(BB·L/W)近交系大鼠的建立并利用此种动物模型探讨了无菌环境对青少年型糖尿病发生的影响。 BB·L/W母鼠经剖腹手术获得无菌仔鼠,保持于无菌环境中至180—210日龄,2/27(7.41％)发生糖尿病;在无菌环境中出生并于断奶后移到常规环境,或在无菌环境中怀孕于常规环境中出生的对照组,9/23(39.13％)发生糖尿病。结果表明,微生物因子,尤其病毒感染在本病的发病机制上起着很大的作用,由于无菌隔离器的屏障作用,无菌BB·L/W大鼠发病率显著降低。7.41％无菌大鼠的发病原因仍有待进一步探讨。

The Effect of Environment Factors on the Development of Juvenile Diabetes in BB·L/W Rats: I. The Effect of Germfree Environment

An inbred rat strain, germfree Lobund BB/W (BB·L/W), was established in this experiment and the effect of germfree environment on the pathogenesis of juvenile diabetes was explored between November, 1982 and January, 1984 at Lobund Lab. BB·L/W female rats were subjected to cesarian section in stainless steel-germfree isolator, pups were fosterparented by germfree Lobund Wistar nursing mother and maintained in germfree environment up to 180--210 days of age. The aver- age age of onset diabetes delayed (157±16 days of age), 2/27 (7. 41%) of germfree BB·L/W rats became diabetic; but in the control group (born in germfree isolators and moved to conventional environment after weanling, or pup's mothers were pregnant in germfree environment but pups were deliYered in conventional cages), 9/23 (39. 13%) came down with diabetes. The incidence of diabetes in the group of germfree rats was decreased strikingly than that in control group. The results indicated that microbiological factors, especially viral infection played a very important role in the pathogenesis of diabetes. The reason why germfree rats came down with diabetes at the rate of 7. 41% still remained unknown.