Estimation of active soil microbial biomass by mathematical analysis of respiration curves: Development and verification of the model |
| |
| Institution: | 1. Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-921, Republic of Korea;2. Research Institutes of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea;3. Department of Animal Life Science, Kangwon National University, Chuncheon, 24341, Republic of Korea;4. Kustogen, Chuncheon, 24341, Republic of Korea;5. Department of Applied Animal Science, Kangwon National University, Chuncheon, 24341, Republic of Korea;1. Department of Biological Sciences, Mailstop 3131, Texas Tech University, Lubbock, TX 79409, USA;2. Johnson, Mirmiran & Thompson, 615 Crescent Executive Court, Suite 106, Lake Mary, FL 32746, USA;3. Texas Tech Climate Science Center, Texas Tech University, 2500 Broadway, Lubbock, TX 79409, USA;1. Department of Agricultural Biotechnology, Centre for Food Safety and Toxicology, Centre for Food and Bioconvergence, Research Institute for Agriculture and Life Sciences, CALS, Seoul National University, Seoul, 08826, Republic of Korea;2. Department of Molecular Biology, College of Natural Science, Pusan National University, Busan, 46241, Republic of Korea;1. Department of Civil Engineering, Hefei University of Technology, Hefei 230009, China;2. Department of Water and Soil Sciences, China Agricultural University, Beijing 100193, China |
| |
| Abstract: | A new method is proposed for estimating the amount of biomass that is capable of directly metabolizing a given substrate. A sample, containing a given amount of active biomass is amended with the substrate and oxygen uptake monitored continuously for 120 h. From this information, supplemented by the substrate removal curve, the time-course of the biomass may be calculated. This calculation is based on a set of four biokinetic equations comprising of the following arbitrary constants: maximum specific growth rate, substrate affinity, biomass growth yield coefficient and biomass maintenance coefficient. In this paper, the validity of the method is verified for liquid axenic cultures and mixed cultures. The method is also shown to be applicable to soils amended with acetate or glucose. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
