山东地方鸡种Mx基因遗传变异与免疫性状的关系

采用PCR SSCP技术研究山东地方鸡种(鲁禽鸡、琅琊鸡、石歧杂鸡、汶上芦花鸡、莱芜黑鸡、济宁百日鸡)Mx基因多态位点与绵羊红细胞(sheep red blood cell, SRBC)免疫抗体滴度和禽流感(avian influenza, AI)、新城疫(newcastle disease, ND)免疫抗体滴度等免疫性状的关系。扩增包含Mx基因intron 13和exon 14约150 bp的片段,通过SSCP分型、测序,在5个地方鸡种共发现2个SNP突变位点分别为Mx基因编码序列第1 892位点G→A(Ser→Asn)，第1 911位点G→A。与免疫性状进行关联分析,发现鲁禽鸡、琅琊鸡、莱芜黑鸡Mx基因编码序列1 892位点与H9抗体滴度显著相关(P＜0.05)。

Associations between immune traits and genetic variation of Mx gene in Shandong indigenous chicken populations

The PCR SSCP was used to study the associations between immune traits(sheep red blood cell, avian influenza and newcastle disease)and polymorphic loci of the Mx gene in Shandong indigenous chicken populations(Luqin chicken, Shi qiza chicken, Langya chicken, Laiwu black chicken and Jining Bairi chicken). The fragment of Mx gene about 150 bp including intron 13 and exon 14 was amplified. The results showed that two single nucleotide polymorphisms were detected in the five indigenous chicken populations by PCR SSCP: 1 892 points of coding sequence of Mx gene G→A (Ser→Asn); 1 911 points G→A, which couldnt lead to amino acid mutation. Through the analysis on the association between the polymorphic loci of the exon 14 and the antibody response, the SNPs on the 1 892 points of coding sequence of Mx gene in Luqin chicken, Langya chicken and Laiwu black chicken was correlated to antibody response of H9 significantly(P＜0.05).