| 灰棕紫泥土脲酶动力学及反应条件研究 |
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| 引用本文： | 李昌满,王贵学,王慧超,许明惠.灰棕紫泥土脲酶动力学及反应条件研究[J].土壤通报,2010(1). |
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| 作者姓名： | 李昌满 王贵学 王慧超 许明惠 |
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| 作者单位： | 重庆大学生物工程学院;长江师范学院生命科学系;重庆工贸职业技术学院; |
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| 基金项目： | 重庆市涪陵区科技项目(kj2007-35)资助 |
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| 摘 要： | 以底物浓度、pH、温度、培养时间作为处理,采用靛酚蓝比色法测定灰棕紫泥土脲酶动力学参数和反应条件。结果表明,灰棕紫泥土脲酶酶促反应V0与S]的关系呈二次线性关系,S]为11.67%时,V0达极大值;Km值在中性、35℃环境下较小,Vmax值在中性环境下较大,与温度呈二次正相关;脲酶酶促反应最适pH值为6.9、最适培养时间为24h、最适底物浓度为10%、最适温度为45℃。
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| 关 键 词： | 土壤 脲酶 动力学 灰棕紫泥 |
Studies on Kinetic and Reaction Conditions of Urase in Gray-brown-purple-mud Soil |
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| LI Chong-man ,WANGGui-xue ,WANGHui-chao ,XUMING-hui.Studies on Kinetic and Reaction Conditions of Urase in Gray-brown-purple-mud Soil[J].Chinese Journal of Soil Science,2010(1). |
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| Authors: | LI Chong-man WANGGui-xue WANGHui-chao XUMING-hui |
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| Institution: | LI Chong-man 1,2,WANGGui-xue 1,WANGHui-chao 2,XUMING-hui 3 (1.College of Bioengineering Chongqing University,Chongqing,400030,China,2.Department of Life Science,Yangtze Normal University,Fuling,408100,3.Chongqing Industry & Trade Polytechnic,Chongqing Fuling 408000,China.) |
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| Abstract: | The kinetic parameters and reaction conditions of urease were studied in gray-brown-purple-mud soil using indophenol blue colorimetry under different conditions,such as substrante concentration,pH value,temperature,culture time.The results indicated that urase reaction had a quadratic linear relation between the V 0 and S] in gray-brown-purple-mud soil.V 0 reached a maximum when S] was 11.67%.Km reached a minimum in neutral soil environment and at 35 ℃.Vmax reached a maximum in neutral soil environment,which had a quadratic positive correlation with temperature.The optimum conditions of urase reaction were at pH 6.9,45 ℃,24h and 10% of substrate concentration. |
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| Keywords: | Soil Urase Kinetics Gray-brown-purple-mud soil |
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