MSTN基因在不同月龄滩羊不同肌肉组织中的定量表达研究

目的]研究MS删基因在不同月龄滩羊不同肌肉组织中的表达差异。方法]根据GenBank已发表的绵羊MSTN基因序列（NM001009428．1）设计l对引物，以滩羊肌肉组织cDNA为模板，采用实时荧光定量PCR技术（Real—timePCR）检测MS7Yv基因在不同月龄滩羊的背最长肌、腿肌和胸肌中的表达量。结果]MS驯基因在背最长肌中的表达量最高，其次为胸肌，腿肌中的表达量最低；随着月龄的增加，滩羊3种肌肉组织中的表达量均呈先升高、后降低、再升高的趋势。结论]不同月龄滩羊肌肉中的MSTNmRNA表达量存在差异．这些差异可能会影响其生长发育和产肉性能等指标。

Quantitative Research on Expression of MSTN Gene in Different Tissues of Tan Sheep with Different Ages

Objective ] The aim of the present study was to investigate the differences in MSTN gene expression in different tissues of Tan sheep with different ages. Methods] According to the published MSTN gene sequence of sheep （NM_001009428.1） in GenBank, a pair of specific primers was designed to amplify the part of cDNA sequence of MSTN using RT-PCR technique. The relative expression level of MSTN gene in longissimus dorsi, leg muscle and breast muscle of Tan sheep with different ages were analyzed by Real-time PCR. Results ] The results showed that the highest expression of MSTN gene were found in longissimus dorsi, followed by breast muscle and leg muscle; with the increase of age of month, the expression of MSTN gene in three kinds of muscle tissues showed a tendency that firstly increased, then decreased and eventually increased. Conclusions] There were differences of MSTN mRNA expression in muscle tissues of Tan sheep with different ages, which might impact on their growth and development as well as meat production performance.