Molecular assay of novel pasturage, sugarcane-grass 94-42, using RAPD markers

Sugarcane-grass 94-42, a novel pasturage, was derived from the cross of a sugarcane cultivar line Co419 and a wild line PT43-52 (Saccharum robustum Brand. et Jesw. ex Grassl). To assay the genetic diversity among Co419, PT43-52 and the F₁ hybrid sugarcane-grass 94-42, 67 RAPD markers were used to detect the genomes of three lines. Around 284 bands were produced by amplification of which 116 were shown to be polymorphic in three lines. Homology of the three lines was analyzed based on all bands. Results showed that the similarity coefficient between Co419 and PT43-52, PT43-52 and sugarcane-grass 94-42, and Co419 and sugarcane-grass 94-42 were 0.7658, 0.8009 and 0.9138, respectively. A total of 95.4% bands amplified from sugarcane-grass 94-42 were homologous with those from either Co419 or PT43-52, which proved that sugarcane-grass 94-42 was the filial generation of Co419 and PT43-52.