台湾黑僵菌防治海榄雌瘤斑螟的菌株筛选

应用9株台湾黑僵菌菌株,筛选出对海榄雌瘤斑螟(Acrobasis sp.)具有高效防治的菌株,并开展林间应用研究。结果表明:在使用浓度为1×108孢子·m L-1的条件下,不同黑僵菌菌株对海榄雌瘤斑螟幼虫具有显著不同的致病力,其中黑僵菌F061菌株的致病力最强,在12 d的累积死亡率达98.75%,校正死亡率达97.56%±1.24%,均极显著的高于其他处理组(P<0.01),且对海榄雌瘤斑螟幼虫的致死中时最短,为7.25 d。在林间应用中,以1×109孢子·m L-1和2×109孢子·m L-1黑僵菌F061菌株处理对海榄雌瘤斑螟的防治效果最好,分别达到90.03%±1.74%和90.55%±1.21%,均极显著的高于其他处理(P<0.01)。从海榄雌瘤斑螟的防治效果结和应用成本考虑,选取1×109孢子·m L-1黑僵菌F061菌株可达到较好的防控效果。

Selection of high efficient strains of Metarhizium anisopliae to control Acrobasis sp

From Taiwan, 9 Metarhizium anisopliae strains were introduced to controll mangrove Acrobasis sp. and effects of forest application were compared. The results found that: ( 1) The pathogenicity of the 9 strains was different to controll Acrobasis sp. larvae, but F061 strains was the strongest as the death rate of Acrobasis sp. was 98.75% and the corrected death rate in laboratory 12 d was 97.56%±1.24%, which were significantly higher than other treatments (P< 0.01), and the LT50 was 7.25 d that was the shortest. (2)The best control effect of Acrobasis sp., for the field application, was 1×109 spore·mL-1 and 2×109 spore·mL-1 of Metarhizium anisopliae F061 strains in mangrove, which were 90. 03%± 1. 74% and 90. 55%± 1. 21%, respectively, and were significantly higher than other treatment group (P < 0.01). Finally, the 1×109 spore·mL-1 of F061 strains was suggested from the analysis of control effect and application cost.