| 高产纤维素酶菌株的鉴定及其活性特征 |
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| 引用本文： | 简立燕,何东进,刘进山,洪伟,游巍斌,肖石红.高产纤维素酶菌株的鉴定及其活性特征[J].福建林学院学报,2017,37(2). |
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| 作者姓名： | 简立燕 何东进 刘进山 洪伟 游巍斌 肖石红 |
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| 作者单位： | 1. 福建农林大学林学院,福建福州,350002;2. 天宝岩国家级自然保护区,福建永安,366032 |
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| 基金项目： | 国家自然科学基金项目，教育部博士科学点基金项目，福建省自然科学基金项目 |
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| 摘 要： | 为分析倒木中具有高产纤维素酶菌株的羧甲基纤维素(CMC)酶活性的大小,以天宝岩国家级自然保护区内长苞铁杉倒木作为研究对象,将菌源通过富集培养、滤纸崩解、CMC酶活测定,分离筛选出具有高效降解纤维素的菌株,并将筛选出的高产纤维素酶菌株进行18S rRNA测序分析鉴定。结果表明,经过初筛、复筛,得出TB4为高产纤维素酶的目的菌株,经鉴定该菌株为小刺青霉,该菌株产纤维素酶的酶活性为243.73 U·mL~(-1)。
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| 关 键 词： | 纤维素降解菌 酶活性 倒木 长苞铁杉 |
Identification of strains producing cellulase and its enzyme activity determination |
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| JIAN Liyan,HE Dongjin,LIU Jinshan,HONG Wei,YOU Weibin,XIAO Shihong.Identification of strains producing cellulase and its enzyme activity determination[J].Journal of Fujian College of Forestry,2017,37(2). |
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| Authors: | JIAN Liyan HE Dongjin LIU Jinshan HONG Wei YOU Weibin XIAO Shihong |
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| Abstract: | Based on cumulation cultivation,filter paper disintegrating,carboxymethyl cellulose (CMC) enzyme activity determination,strains with great capacity in cellulose decomposition were isolated to analyze the CMC enzyme activities of fallen logs in the Tsuga longibracteata W.C.Cheng forest of Tianbaoyan National Nature Reserve.18S rRNA sequencing analysis of the selected stains was carried out and identified as Penicillium spinulosum which the CMC enzyme activity was 243.73 U·mL-1.The P.spinulosum strain had the ability in cellulose degradation in the T.longibracteata forest of Tianbaoyan National Nature Reserve. |
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| Keywords: | cellulose-decomposing microorganisms enzyme activity fallen log Tsuga longibracteata W C Cheng |
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