Interspecific hybridization in Gossypium L.: characterization of progenies with different ploidy‐confirmed multigenomic backgrounds |
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Authors: | Ganesh S. Newaskar Vivek P. Chimote Subhash S. Mehetre Ashok S. Jadhav |
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Affiliation: | 1. State Level Biotechnology Centre, Mahatma Phule Krishi Vidyapeeth, , Rahuri, 413 722 India;2. Office of Directorate of Research, Mahatma Phule Krishi Vidyapeeth, , Rahuri, 413 722 India |
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Abstract: | Cytological and molecular investigations were undertaken for parent and progeny derived from a trispecific line [2(Gossypium arboreum × G. anomalum) × G. hirsutum var. BWR], which was crossed with G. hirsutum var. JLH168. Cytomorphological analysis of the F1 (G. arboreum × G. anomalum), its amphidiploid and progeny from trispecies hybrid showed distorted ploidy segregation with monovalents to hexavalents and high intergenomic (small A2 and large B1) allosynthetic chromosome pairing. Microsatellite analysis identified three fragments associated with G. arboreum and G. anomalum and six fragments associated with G. hirsutum in derivates of the trispecies line × G. hirsutum var. JLH168. Inter‐Retrotransposon Amplified Polymorphism (IRAP) analysis revealed fragments of G. arboreum and G. anomalum, only in F1 and amphidiploid. Chromosomal association and microsatellite analysis of three progeny genotypes (i.e. haploid, hexaploid and tetraploid no. 1) confirmed that they share multigenomic background from the three cotton species (A2, AhDh and B1 genome). The interspecific hybrid cotton genotypes studied are likely to be useful for the introgression of genes from diploid species to commercial upland cultivars. |
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Keywords: | cytology
Gossypium
interspecific hybridization IRAP markers microsatellite markers |
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