Multiple forms of acetyl-naphthyl-esterase activity in soil organic matter |
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Authors: | G. Cacco A. Maggioni |
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Affiliation: | Institute of Agricultural Chemistry, University of Padua, Italy |
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Abstract: | Acetyl-naphthyl-esterase activity has been identified and characterized in organic matter extracted from an A1 horizon of an Alpine podzol. The temperature optimum of the esterase is about 75° C and its activity rises with increasing pH, without reaching a maximum value in the tested range. The Michaelis constant has been determined as Km = 2.950 mM. Pronase does not disrupt esterase activity. Electrofocusing in acrylamide gel shows several peaks of enzyme activity, which correspond with humic isoelectric bands. The location of acetyl-esterase activity in organic matter is discussed. |
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