| DDT降解菌DH-7的分离鉴定与降解特性研究 |
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| 引用本文： | 李红权,马珦玻,李红梅,昌艳萍,赵盛,陈淑兰,周艳春. DDT降解菌DH-7的分离鉴定与降解特性研究[J]. 安徽农业科学, 2009, 37(13): 5851-5852 |
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| 作者姓名： | 李红权 马珦玻 李红梅 昌艳萍 赵盛 陈淑兰 周艳春 |
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| 作者单位： | 1. 河北大学基础医学院,河北保定,071000
2. 河北大学生命科学学院,河北保定,071002 |
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| 基金项目： | 河北省教育厅基金(2008309); 河北省自然科学基金(C2007-000188) |
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| 摘 要： | [目的]研究DDT降解菌的生物学、降解特性及其发酵条件的优化。[方法]从化工厂采集土样，分离、筛选到1株能够在好氧条件下DDT降蟹率较高的菌株DH-7，并对其进行研究。[结果]通过16SrDNA序列分析结合传统分类学方法初步确定菌株DH-7为铜绿假单胞菌。对菌体降解DDT特性的研究表明，该菌株对DDT降解10d的降解率为73．6％。在优化培养条件后，该菌株10d的降解率达81．4％。[结论]该研究结果为DDT污染土壤的生物修复提供依据。
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| 关 键 词： | 铜绿假单胞球菌 DDT降解 16S rDNA |
Study on Isolation and Characterization of a DDT Degradation Bacterium Strain DH-7 |
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| LI Hong-quan et al. Study on Isolation and Characterization of a DDT Degradation Bacterium Strain DH-7[J]. Journal of Anhui Agricultural Sciences, 2009, 37(13): 5851-5852 |
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| Authors: | LI Hong-quan et al |
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| Affiliation: | LI Hong-quan et al (School of Basic Medical Science,Hebei University,Baoding,Hebei 071000) |
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| Abstract: | [Objective]The purpose of this research was to study biological characteristics,degradation characteristics and the optimization of fermentation conditions of DDT degradation bacterium.[Method] A bacterium strain DH-7 having capability of degrading DDT was isolated from the DDT contaminated soil of a chemical factory.The characteristics of DH-7 were studied.[Result]Based on the phenotype,physiological and biochemical characteristics,and the phylogenetic analysis of 16S rDNA sequence,the strain DH-7 was iden... |
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| Keywords: | Pseudomonas aeruginosa DDT degradation 16S rDNA |
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