动物细胞大规模培养技术研究进展

利用动物细胞大规模培养技术可生产多种生物制品,为提高细胞活力和细胞生长密度,采用有多种添加成分的无血清培养基培养细胞,选择既有利于细胞生长又可提高培养细胞密度的微载体和条件温和、易操作、气体交换速度快的生物反应器,在线监控细胞生存环境和生理活动,减少培养过程中培养基的抑制因素,从而给细胞提供更好的生存环境;另外通过向细胞中导入抗凋亡基因,可减缓细胞凋亡的发生,提高细胞活性和蛋白产量;此外,在动物细胞大规模培养过程中,利用多孔微载体能高效、大量地增殖细胞,具有良好的应用前景.

Research advance in large - scale culture of animal cells

Many biological products were manufactured by means of large - scale culture of animal cells. To increase cell - specific productivity and cell density, serum - free media supplemented with several nutriments were used for cell culture, and microcarriers were chosen in favor of cell growth and high cell density. Bioreactors with simple manipulation, good qualification and aeration were adopted. More suitable conditions for cell growth could be given through on- line supervising the environment for cell and restraint factors in cell culturing. Furthermore, the anti - apoptosis genes using recombinant DNA technology can increase cell viability and productivity. Porous microcarriers was emphasized in large - scale culture of animal cells.