CO2 与养分交互作用对番茄幼苗养分利用的影响

以番茄(Lycopersicon esculentumMill.)‘合作906’为材料进行溶液培养试验,设2个因子:CO2和营养液浓度;CO2浓度设正常(360μL/L)和倍增(720μL/L)2个水平;营养液浓度设基本营养液(日本山崎番茄营养液),微量元素采用阿农营养液配方的1/2、1/4、1/8、1/164个水平,完全试验方案8个处理,3次重复。pH为6·0±0·2,3d更换1次营养液。移植到1·2L盆(2株/盒)中,植株在CO2生长箱(VS-3DMC)中培养,全天施放CO2,白天25℃,晚上15℃,光照为14h/d,光照强度11000lx,相对湿度60%。46d时收获,根、茎、叶经蒸馏水冲洗吸干水分后,放入纸袋105℃杀青,75…

Effect of Carbon Dioxide Enrichment Interactions with Nutrient Concentration on Nutrient Use Efficiency of the Tomato Seedlings

AIM: To explore the molecular mechanism of anti-shock effect of Shenmai (SM) injection and to observe its influence on TLR4 and IκB mRNA expression in hemorrhagic shock (HS) plus LPS rat lung. METHODS: Rats were divided into HS, HS+LPS, HS+LPS+ dexamethasone (DEX), HS+LPS+SM group, respectively. HS+LPS injury model of rats were induced by hemorrhagic shock (MAP 40 mmHg for 10 min) followed by intravenous administration of lipopolysaccharide (LPS, 1.5 mg/kg). Four hours later, the TNF-α contents in lung tissue were measured by ELISA, TLR4 mRNA and IκBα mRNA expression were assayed by RT-PCR, ultrastructural changes of the type Ⅱ alveolar cell were observed by electron microscope. RESULTS: The TLR4 mRNA expression level in HS+LPS+SM group was significantly decreased as compared to HS+LPS group (P<0.05). Otherwise, the level of IκBα mRNA expression in HS+LPS+SM group was significantly increased as compared to HS+LPS group (P<0.05). The TNF-α content in lung tissue in HS+LPS+SM group was obviously lower than that in HS+LPS group (P<0.05). In HS+LPS+SM group, the lung pathological injuries were alleviated as compared to HS+LPS group. CONCLUSION: Shenmai injection down-regulates TLR4 mRNA expression and up-regulates IκBα mRNA expression in the lung, and inhibits the release of TNF-α in rats exposed to HS and LPS. These results suggest that Shenmai injection might have a protective effect on the tissue injured from hemorrhagic shock and endotoxic shock by regulating NF-κB signal transduction pathway and inhibiting pro-inflammatory cytokine production.