A comparison of molecular methods for monitoring soil nematodes and their use as biological indicators |
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| Authors: | XY Chen TJ Daniell R Neilson V O’Flaherty BS Griffiths |
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| Institution: | 1. Teagasc, Environment Research Centre, Johnstown Castle, Wexford, Ireland;2. Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK;3. Dept Microbiology, National University of Ireland, Galway, Ireland;4. Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | Soil fauna, especially soil nematode communities may be used as indicators for monitoring soil biodiversity and ecological processes. A major drawback facing ecologists is the specialised taxonomic knowledge and labour intensive nature of the work required for traditional morphological identification of soil fauna. We review rapid molecular methods, including: DNA Barcoding or sequencing, PCR-DGGE, PCR-TRFLP and real-time PCR, which could enable an empirical assessment of soil nematode assemblages, in relation to their use as monitoring tools. Based on advantages of: high-throughput; ease of comparison between samples; and rapid data analysis, we argue that PCR-TRFLP is well suited to monitoring purposes. |
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