新疆褐牛产奶性状候选基因SNPs检测及其遗传效应分析

本研究旨在探索新疆褐牛产奶性状相关候选基因多态性与泌乳性状及体细胞评分(SCS)的相关性,探究新疆褐牛多态性位点的群体遗传特征。基于课题组前期DNA混池重测序的结果筛选17个SNPs突变信息,并采用基质辅助激光电离解吸飞行时间质谱法(Sequenom Mass Array Genotype)分型技术验证FASN、FSHR、HAL、GPIHBP1、NIBPL、C9、PDE9A、PTK2、STAT1、ZNF66、ZNF567、ZNF7、ZNF623和ZNF804A基因在新疆乌鲁木齐种牛场、新疆天山畜牧生物工程股份有限公司和新疆塔城地区种牛场共计262头新疆褐牛母牛群体中的遗传多态性,运用SAS 8.1软件GLM过程分析这14个基因多个SNP位点对新疆褐牛群体的遗传效应及其与305天产奶量、乳脂率、乳蛋白率、乳糖率、总固体和SCS的关联性。结果表明,17个SNPs均处于哈代-温伯格平衡状态(P0.05),9个SNPs与新疆褐牛1个或多个泌乳性状达到显著(P0.005)或极显著(P0.001)关联,这些位点分别位于FASN、FSHR、HAL、ZNF66、ZNF7、ZNF804A、GPIHBP1和PDE9A基因上。提示这些基因可尝试作为新疆褐牛产奶性状分子标记的候选基因,为新疆褐牛产奶性状的分子标记辅助选育提供依据和参考。

Genetic Effect and Detection of SNPs in Candidate Genes Related to Milk Production Traits of Xinjiang Brown Cattle

This study aimed to explore the association between polymorphisms of candidate genes related to milk production traits and somatic cell score (SCS), and explore the genetic feature of polymorphism from these SNPs in Xinjiang brown cattle. Based on DNA pools with the Re-sequencing technology to gain SNPs data, totally screened 17 SNPs of FASN, FSHR, HAL, GPIHBP1, NIBPL, C9, PDE9A, PTK2, STAT1, ZNF66, ZNF567, ZNF7, ZNF623 and ZNF804A genes, at the same time, combining with Sequenom MassArray Genotype technology to detect the genetic polymorphism. The genetic effect of 17 SNPs on 305 d milk yield, milk fat percentage, milk protein percentage, milk lactin percentage, total solids content and SCS at Xinjiang Urumqi farm, Cattle farm in Tacheng area and Xinjiang Tianshan Animal Husbandry Biological Engineering Co., Ltd. were analyzed by GLM procedure of SAS8.1.Totally 262 Xinjiang Brown cattle. The results showed that the 17 SNPs were in Hardy-Weinberg equilibrium, and 9 SNPs had significant (P<0.005) or extremely significant (P<0.001) association with milk production traits, and these SNPs were located in FASN, FSHR, HAL, ZNF66, ZNF7, ZNF804A, GPIHBP1 and PDE9A genes. Association analysis results of this study provide a theoretical basis and reference for the molecular marker-assisted selection in Xinjiang brown cattle.