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黄芩组培工厂化技术研究
引用本文:李永文,寇凤仙,李 红,周艳珍,温秀荣.黄芩组培工厂化技术研究[J].中国农学通报,2007,23(4):71-71.
作者姓名:李永文  寇凤仙  李 红  周艳珍  温秀荣
作者单位:保定职业技术学院农林与生物工程系,河北,071051
基金项目:河北省科技厅项目“药用植物组培工厂化育苗技术研究”(06225509).
摘    要:选用黄芩健壮枝条作为材料,进行组培快速繁殖技术研究。结果表明:愈伤组织诱导激素组合以6-BA1.0mg/L+NAA0.5mg/L最为有利,所形成愈伤组织生长速度和质量均满足要求;不定芽分化激素组合为6-BA1.0mg/L+NAA0.1mg/L,增殖方式为器官发生型再生方式;继代培养采用NAA0.5~0.1mg/L+B90.5~0.1mg/L,增殖在10倍以上;生根培养NAA0.5mg/L+IBA 0.1mg/L,一般15d左右生根,平均根量4~5条,生根率近100%,能显著提高移栽成活率,满足植物组织培养工厂化育苗技术要求。

关 键 词:麻黄  麻黄  组织培养  分子生物学  
修稿时间:2007-02-12

Study on the Industrialized Rapid Clone Propagation Technology of Scutellaria baicalensis Georgi
Li Yongwen,Kou Fengxian,Li Hong,Zhou Yanzhen,Wen Xiurong.Study on the Industrialized Rapid Clone Propagation Technology of Scutellaria baicalensis Georgi[J].Chinese Agricultural Science Bulletin,2007,23(4):71-71.
Authors:Li Yongwen  Kou Fengxian  Li Hong  Zhou Yanzhen  Wen Xiurong
Institution:Baoding Vocational and Technical College, Hebei 071051
Abstract:Selects the vigorous and healthy branch of Scutellaria baicalensis Georgi as explants carried on the industrialized rapid clone propagation technology research. The results showed that plant hormone combination of 6-BA1.0mg/L NAA0.5mg/L is good for callus induction, 6-BA1.0mg/L NAA0.1mg/L is suitable for Adventitious buds, NAA0.5~0.1mg/L B90.5~0.1mg/L is good for cultivation of young plant, the coefficient of reproduction is up to 10. NAA0.5mg/L IBA0.1mg/L is best for rootage, rooting rate was 100%, and completely meets the demands of the industrialized rapid clone propagation technology.
Keywords:Scutellaria baicalensis Georgi  plant tissue culture  rapid clone propagation
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