新疆两泥火山可培养中度嗜盐微生物多样性

[目的]了解新疆乌苏和独山子两泥火山可培养中度嗜盐菌的多样性.[方法]采用10;NaCl盐浓度的ISP5培养基,对两泥火山样品进行可培养菌分离、16S rDNA鉴定及系统发育分析.[结果]从新疆两泥火山中共分离得到26株中度嗜盐细菌和放线菌,乌苏泥火山13株,分属于7个属:Nesterenkonia、Bacillus、Salinicoccus、Nocardiopsis、Halomonas、Halophilic和Micrococcacea,其中Nesterenkonia属为优势菌群;独山子泥火山13株,隶属于6个属:Halomonas、Marinobacter、Bacillus、 Salinicoccus、Nocardiopsis和Halolactibacillus,其中Halomonas和Marinobacter属为优势菌群.4株可能为潜在的新种.[结论]新疆两泥火山可培养中度嗜盐细菌和放线菌具有较为丰富的生物多样性,且潜藏着新的菌种资源,具有进一步研究开发价值.

Diversity of Cultivable Moderately Halophilic Microbes from Two Mud Volcanoes in Xinjiang

[Objective] In order to understand the diversity of cultivable moderately halophilic bacteria from two mud volcanoes in Wusu and Dushanzi,Xinjiang.[Method]The separation of cultivable microbes based on the samples from the two mud volcanoes,detection of 16S rDNA and analysis of systematical developoment were conducted with 10% NaCl concentration.[Result] A total of twenty-six moderately halophilic bacteria and actinomyces were isolated from the two mud volcanoes in Xinjiang.Thirteen strains from Wusu mud volcano grouped into seven genera,including Nesterenkonia,Bacillus,Salinicoccus,Nocardiopsis,Halomonas,Halophilic and Micrococcacea.Thirteen strains from Dushanzi mud volcano belonged to six genera,including Halomonas,Marinobacter,Bacillus,Salinicoccus,Nocardiopsis and Halolactibacillus.Nesterenkonia was typical of the Wusu mud volcano sample,while Halomonas and Marinobacter prevailed in the Dushanzi mud volcano sample.Additionally,four poteneial novel species were found.[Conclusion] The cultivable moderately bacterial population diversity is abundant in these two mud volcanoes.In addition,there are potential microbial resources in this environment,which is worth further studying and exploitating.