高秆野生稻(Oryza alta L.)叶片组织培养中通过胚状体获得再生植株

高秆野生稻叶片可诱导出胚性和非胚性2种愈伤组织。胚性愈伤组织在合适的分化培养基上可不断形成胚状体,进而大量获得再生植株,再生植株以绿苗为主,未见绿白嵌合苗。源于不同脱分化培养基的胚性愈伤组织具有相似的胚状体形成和植株再生能力。诱导胚性愈伤组织以N6培养基添加NAA1 mg/L、2,4-D2 mg/L和ZT0.5 mg/L效果最好,形成胚状体并分化植株以MS培养基添加NAA2 mg/L和IAA0.5 mg/L为佳。

Gaining regeneration plantlets from embryoids in leaf tissue culture of Oryza alta L.

The leaves of Oryza alta could be induced to form embryonic and non-embryonic calli, and the embryonic calli cultured on appropriate differential media could be induced to form embryoids from which large number of regeneration plantlets could be obtained. Most of regeneration plantlets were green and there were no green-white mosaic plantlets among them. The embryonic calli from different dedifferentiation media had nearly the same ability of the embryoid formation and plantlet regeneration. The N6 medium supplemented with 1 mg/L NAA, 2 mg/L 2,4-D and 0.5 mg/L ZT had the best effect on embryonic callus formation, and the MS medium supplemented with 2 mg/L NAA and 0.5 mg/L IAA was the best for the embryoid formation and the plantlet regeneration.