| Callus initiation and subculture of Taxus chinensis |
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| 引用本文： | 李家儒,刘曼西,陈辉蓉,吴振斌,王君健. Callus initiation and subculture of Taxus chinensis[J]. 林业研究, 1999, 0(1) |
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| 作者姓名： | 李家儒 刘曼西 陈辉蓉 吴振斌 王君健 |
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| 作者单位： | 1. lnstitute of Hydrabiology，The Chinese Academy of Sciences，Wuhan 430072，P. R. China2. Department of Bioengineering，Huazhong University of Science and Technology. Wuhan 430074，P. R. China |
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| 摘 要： | IntroductionTaxolisacompIexditerpenoidsecondaryproductofthegenushauSthatwasapprovedfortreatmentagainstovarianandbreastcancersandshowsprom-iseagainstothercancers.DuetotherelativescarcityofthefewnaturaIresourcesandthelowyieldoftaxol,thesupplyoftaxolisrestricted,limitingexpansionofcIinicaItrialsandtreatmentavailabiIity.lnterestinalternativemethodsfortaxoIproductionhasbeenintensifying.CellcuItureprovideaconvenientsystemforstudyingthebiosynthesisoftaxol.Itmaybeaviablealternativefortaxolproduct(…
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Callus initiation and subculture of Taxus chinensis |
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| Li Jiaru, Liu Manxi, Chen Huirong,Wu Zhenbin, Wang Junjian. Callus initiation and subculture of Taxus chinensis[J]. Journal of Forestry Research, 1999, 0(1) |
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| Authors: | Li Jiaru Liu Manxi Chen Huirong Wu Zhenbin Wang Junjian |
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| Abstract: | Conditions have been established for the callus initiation and subculture of T chinensis. The calliwere induced by the explants cultured first on the medium MS supplemented with 1 .0 mg/L 2,4-D, 2g/L CH, and 25g/L sucrose, then on The medium f MS+1 .0 mg/L NAA+0.5 mg/L BA+2 g/L CH+25 g/L sucrose. When the callus was Subcultured and tamed several times, it could grow fast and stable on the medium : MS+0.2mg/L 2,4-D+0.5mg/L NAA+0.5 mg/L BA+2 g/LCH+25 g/L sucrose. The contamination of explants was a result of endophytic microbes of T Chinensis. This could be avoided by adopting the tender shoots 3-5 cm long collected in early spring as the source of explants. The browning of the Cultures could be prevented and controlled by means of the selection of a suitable explants, hormonal regime in the medium, culture methods and the use of antioxidants. |
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| Keywords: | Taxus chinensis tissue culture taxol |
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