正交设计在多年生黑麦草组织培养中的应用

以多年生黑麦草的成熟种子为外植体,应用正交设计方法,对愈伤组织的诱导和分化培养基进行了优化筛选。结果表明,愈伤组织诱导的最佳因素组合:2,4-D(7mg/L) 6-BA(0.1mg/L) NAA(0.3mg/L) CH(0.1g/L)(特拉华);2,4-D(7mg/L) 6-BA(0)(尤文图斯、托亚)。特拉华愈伤组织分化的最佳因素组合:6-BA(0.1mg/L) NAA(0.8mg/L) ZT(0.1mg/L) Cu2 (8.96mg/L);6-BA为0.2mg/L时,托亚的愈伤组织分化率最高。比较三个品种的出愈率和分化率:特拉华最高,托亚次之,尤文图斯最低。

Application of Orthogonal Design to Tissue Culture of Perennial Ryegrass

Mature seeds of three perennial ryegrass (Lolium perenne) varieties were cultured for callus induction and differentiation on MS basic medium containing different concentrations of 2,4-D, 6-BA , NAA and so on. The results showed that there were significant influences of different concentrations of the supplements on callus induction and differentiation. For Delaware, 7mg/L 2,4-D,0.1mg/L 6-BA, 0.3mg/L NAA and 0.1g/L CH is propitious to callus induction. For Juventus and Taya callus induction, 7mg/L 2,4-D and 0 mg/L 6-BA is more favorable. As for callus differentiation,the best ingredients and concentrations for Delaware are 0.1mg/L 6-BA, 0.8mg/L NAA, 0.1mg/L ZT and 8.96mg/L Cu~(2 ). (0.2mg/L) 6-BA is propitious to Taya callus differentiation.In general, Delaware proved to be the best of the three, Taya comes second and Juventus the worst.