| 猪PR-39基因的克隆及在大肠杆菌中的串联表达载体的构建 |
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| 引用本文： | 扈进冬,吴远征,杨合同.猪PR-39基因的克隆及在大肠杆菌中的串联表达载体的构建[J].中国畜牧兽医,2008,35(12):56-59. |
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| 作者姓名： | 扈进冬 吴远征 杨合同 |
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| 作者单位： | 1.山东省科学院中日友好生物技术研究中心，山东省应用微生物重点实验室， 济南 250014;2.山东省科学院生物所， 济南 250014； 3.山东理工大学生命科学院， 淄博 255049 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 采用重叠区扩增法人工合成了大肠杆菌偏爱密码子的猪PR 39基因片段，并克隆至pMD18T中，经测序确认正确后，与pET Trx构建了串联表达载体pET-Trx-(PR-39)3，并在大肠杆菌BL21 (DE3)中诱导获得表达。为PR-39的进一步开发与应用打下了基础。
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| 关 键 词： | PR-39 串联 载体构建 |
Cloning of Gene PR-39 from Pig and Construction of PR-39 as Tandem Repeats Expression Vector in E coli |
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| HU Jin-dong,WU Yuan-zheng,YANG He-tong.Cloning of Gene PR-39 from Pig and Construction of PR-39 as Tandem Repeats Expression Vector in E coli[J].China Animal Husbandry & Veterinary Medicine,2008,35(12):56-59. |
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| Authors: | HU Jin-dong WU Yuan-zheng YANG He-tong |
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| Institution: | 1.Biotechnology Center of Shandong Academy of Sciences,Key Laboratory for Applied Microbiology of Shandong Province,Jinan 250014,China; 2.Biology Institute of Shandong Academy of Sciences,Jinan 250014,China;3. School of Life Sciences,Shandong University of Technology,Zibo 255049, China |
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| Abstract: | Gene RP-39 from pig with the partiality of E coli was synthesized by the method of gene splicing by overlap extension.The fragment was cloned into pMD18-T and sequenced.Three copies of gene RP-39 were combinated into express vector pET-Trx.Then pET-Trx-(PR-39)3 was transformed into E coli BL21(DE3) and RP-39 was inducted which established good foundation for the future development. |
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| Keywords: | gene synthesis PR-39 tandem repeats construction of expression vector |
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