野桑蚕乙酰胆碱酯酶1型基因(Bmmace1)5′端非翻译区序列的剪切方式分析

通过5′cDNA末端快速扩增(RACE)的方法,克隆了野桑蚕乙酰胆碱酯酶1型基因(Bmmace1)的cDNA 5′端非翻译区序列(5′UTR)区域,与家蚕乙酰胆碱酯酶1型基因(Bmace1)的cDNA 5′UTR(242个碱基)进行比较分析表明,Bmmace1的5′UTR包含有231个碱基,存在2个位点的点突变和一段连续11个碱基的缺失。通过克隆和测序分析野桑蚕基因组中Bmmace1的5′UTR片段,表明Bmmace1和Bmace1基因的RNA剪切都遵循GT-AG规则,但在其cDNA 5′UTR的RNA剪接过程中可能存在差异,Bmmace1在其cDNA上游+33处存在比Bmace1少剪接的序列TGATTTGAAGG,而在其基因组中5′UTR的TGATTTGAAGG序列下游-4位点缺失ACAGA序列。研究结果可为深入探讨Bmmace1基因与野桑蚕产生抗药性的关系提供基础信息。

Splicing Pattern of 5'UTR of Acetylcholinesterase 1 From Bombyx mandarina M.

The 5′ untranslated region(5′ UTR) of acetylcholinesterase 1 gene(Bmmace1) was cloned from Bombyx mandarina through 5′RACE method.The comparison of 5′UTRs between Bmmace1 and Bombyx mori acetylcholinesterase 1(Bmace1) showed that the 5′UTR of Bmmace1 is 231 bp in length,which is shorter than that of Bmace1(242 bp),and there are 2 nucleotide mutations and a loss of 11 consecutive base pairs in 5′UTR of Bmmace1.Cloning and sequencing of the genome sequence containing 5′ UTR of Bmmace1 indicated that both Bmmace1 and Bmace1 follow the GT-AG rule in their exon-intron boundaries,but there are some differences in their splicing patterns in 5′ UTRs.The genome sequence of Bmmace1 contains the lost 11 consecutive base pairs(TGATTTGAAGG) for Bmace1 at +33 site of Bmmace1 cDNA sequence,but there exists a loss of five nucleotides(ACAGA) at-4 site compared to TGATTTGAAGG nucleotides.This research provides some basic information for understanding the relationship between Bmmace1 gene and insecticide resistance.