灰葡萄孢菌不同发酵方式次生代谢产物的生物活性

对灰葡萄孢菌固体和液体发酵后的次生代谢产物乙酸乙酯粗提物进行抑菌、杀虫活性研究,结果表明:用于测试的5种真菌病原菌中,固体发酵粗提物对烟草赤星病菌和稻瘟病菌的菌丝生长有很高的抑制活性,对番茄早疫病菌、菜豆炭疽病菌、梨黑星病菌和稻瘟病菌的孢子萌发有很高的抑制活性;液体发酵粗提物对番茄早疫病菌、梨黑星病菌的菌丝生长和菜豆炭疽病菌的孢子萌发有非常高的抑制率.对2种细菌病原菌烟草野火病菌和白菜软腐病菌,固体、液体发酵粗提物抑制生长率均高达80%以上,但固体稍高于液体发酵粗提物;在杀虫方面,固体发酵粗提物对桃蚜触杀作用明显高于液体发酵粗提物.

Study on Antifungal and Insecticidal Activities of Metabolites of Botrytis cinerea Produced by Different Fermentation Methods

The bioactivities of coarse extracts of the metabolites produced from both solid culture and liquid culture of Botrytis cinerea were studied.The results showed that the extract from solid culture had strong inhibiting effect on hypha growth of Alternaria alternate and Pyricularia oryzae out of the 5 tested pathogen fungi,and it also had evident inhibiting effect on spore germination of Alternaria solani,G.lindemuthianum,Venturia nashicola and Pyricularia oryzae.Whereas the extract from liquid culture could significantly inhibited the hypha growth of Alternaria solani,Venturia nashicola and also inhibited spore germination of G.lindemuthianum.The inhibition rates of the extracts from both solid and liquid culture against two species of tested bacteria of Pseudomonas syuringae pv.tabaci and Venturia nashicola,were over 80%,though the extract from solid culture had a little higher inhibition rate against these bacteria.Furthermore,the extract from solid culture also had higher insecticidal activity against M.persiace,than that of from liquid culture.