Investigation of biological properties, double-stranded RNA patterns and antigen concentration in citrus species infected with citrus tristeza virus |
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Authors: | S Korkmaz |
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Institution: | (1) Dept. of Plant Protection, Faculty of Agriculture, University of Canakkale Onsekiz Mart, 17100 Canakkale, Turkey |
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Abstract: | Biological properties and dsRNA patterns of one Cyprus and three Turkish isolates of citrus tristeza virus (CTV) were investigated.
In addition, CTV antigen concentration and effect of tissue sampling time from naturally infected Shamouti sweet orange trees
grown in the field of Icel Province, Turkey, were also determined. The Cyprus isolate showed vein clearing symptoms on grapefruit,
‘Madam Vinous’ and Mexican lime and stem pitting symptoms on Mexican lime. The three Turkish isolates showed only vein clearing
symptoms on Mexican lime. All four isolates showed a full-length major double-stranded RNA (dsRNA) band of 13.3 × 106 Da mol. wt in extracts from infected Madam Vinous sweet orange trees, and major or minor dsRNA bands with 2.0. 0.8 and 0.5
× 106 mol.wt. All seven different citrus varieties inoculated with the Igdir (D) strain contained full-length dsRNA. The additional
two dsRNA of 0.8 and 0.5 × 106 mol.wt were also detected as clearly as full-length dsRNA in these hosts, but were weaker inCitrus exelsa and ‘Interdonat’ lemon. Madam Vinous, rough lemon and Mexican lime were the best hosts for dsRNA analysis. ELISA values were
highest in April (OD405nm
=0.476), decreased steadily until August, and then increased gradually through December. ELISA values were lowest in July
and August (OD405nm
=0.157 and 0.141, respectively). dsRNA recovery from a field tree infected with isolate Igdir D was good in March, April
and May and poor in January and February. No dsRNA band was detected in August or September.
http://www.phytoparasitica.org posting July 9, 2002. |
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Keywords: | Double-stranded RNA extraction electrophoresis antigen concentration |
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