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| A型产气荚膜梭菌的分离及鉴定 | |
| 引用本文： | 高文伟,郭宏伟,任家琰,蒋玉文,杨茂荣,赵玉臣,柴奎强,周县利.A型产气荚膜梭菌的分离及鉴定[J].中国农学通报,2007,23(7):28-28. |
| 作者姓名： | 高文伟 郭宏伟 任家琰 蒋玉文 杨茂荣 赵玉臣 柴奎强 周县利 |
| 作者单位： | 1. 山西农业大学动物科技学院,太谷,030801 2. 中国兽药监察所,北京,100081 3. 山西省药物培植场,降县,043600 |
| 基金项目： | 山西省攻关项目“鹿出血性肠炎多价疫苗的研制”（012029）. |
| 摘 要： | 从山西各鹿场采集鹿出血性肠炎急性病死鹿病料39例，通过病原微生物分离培养，形态学观察和血清型鉴定，初步表明分离到的产气荚膜梭菌主要是A型，对 PCR产物的序列分析表明经过PCR得到了特异性的α毒素基因片段，因而A型产气荚膜梭菌是引起山西鹿场发生鹿出血性肠炎的主要致病菌。为该疾病的防治和疫苗研制提供依据。 |
| 关 键 词： | N-糖基化人源化 N-糖基化人源化 生物反应器 药用蛋白 转译后修饰 |
| 修稿时间： | 2007-03-222007-04-18 |
Isolation and characterization of type A Clostridium perfringens |
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| Gao Wenwei,Guo Hongwei,Ren Jiayan,Jiang Yuwen Yang Maorong,Zhao Yuchen,Cai Kuiqiang,ZhouXianli.Isolation and characterization of type A Clostridium perfringens[J].Chinese Agricultural Science Bulletin,2007,23(7):28-28. | |
| Authors: | Gao Wenwei Guo Hongwei Ren Jiayan Jiang Yuwen Yang Maorong Zhao Yuchen Cai Kuiqiang ZhouXianli |
| Institution: | 1.College of Animal Science , Shanxi Agricultural University, Taigu 030801;2.The Animal Medication Supervision A cadeny, Beijing 100081; 3.Shanxi Medication Cultivation Field, Jiangxian 043600 |
| Abstract: | Samples were collected from 39 cases of cervus hemorrhagic enteritis in Shanxi province,the major microbial pathogen were isolated and examined microscopically,then identified through serum reaction,the preliminary result showed it is type A Clostridium perfringens,the result were further confirmed by PCR and sequence analysis.So the conclusion was made that type A Clostridium perfringens is the major pathogen that causing cervus hemorrhagic enteritis in Shanxi province,and therefore provide basis for the disease prevention and vaccine development. |
| Keywords: | Shanxi cervus farm type A Clostridium perfringens isolation characterization |
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