桑树硬枝扦插生根能力及其生根关联酶活性的研究

采用鲁桑实生苗、大鲁桑和农桑14号为材料,研究了桑树的扦插生根能力、生长素对扦插生根的影响和生根过程中相关氧化酶的活性。结果表明,鲁桑实生苗的扦插生根效果较好,生根率可达71.1%,而大鲁桑和农桑14号嫁接苗插条的生根率分别为32.20%、44.43%,属插条难生根型品种;用150 mg/L吲哚丁酸(IBA)处理农桑14号,插穗生根率达81.1%,比对照提高了36.67%;桑树硬枝扦插生根过程中,插条内吲哚乙酸氧化酶(IAAO)的活性先升高后降低,过氧化物酶(POD)和多酚氧化酶(PPO)活性逐渐升高,3种酶活性的动态变化与生根过程密切相关。

Rooting Capacity and Correlative Enzymes Activities of Hardwood Cuttings of Mulberry

Rooting capacity and changes of the related oxidase activities of hardwood cuttings from 3 mulberry varieties,Lusang seedling,Dalusang and Nongsang 14,were investigated in this paper.The experiment results showed that cuttings from Lusang seedling has high rooting capacity,and the rooting percentage reached to 71.1%.The rooting percentage of cuttings from Dalusang and Nongsang 14 were only 32.20% and 44.43%,respectively.But the rooting percentage of cuttings from Nongsang 14,treated with 150 mg/L IBA solution,reached to 81.1%,which increased 36.67% higher than that of the control.The activity of indoleacetic acid oxidase increased sharply during induction rooting phase and declined during root expression and elongation phase.The peroxidase and polyphenol oxidase activities increased gradually during rooting phase.The above results suggsted that changes of activities of 3 oxidases tested were tightly related to the rooting process of cuttings in mulberry.