~(137)Cs γ射线与叠氮化钠复合处理水稻的诱变效应

用0、50、100、150、200、250和300Gy的γ射线及0、1、2、3mM的叠氮化钠复合处理水稻品种广陆矮4号的干种子。结果表明,M_1的损伤效应随γ射线剂量和叠氮化钠浓度的提高而增加。复合处理在苗高、根长、苗高活力指数和根长活力指数方面表现累加效应,在染色体畸变方面存在协同作用。复合处理的最佳剂量组合为200Gyγ射线+2mM叠氮化钠,其叶绿素、抽穗期和株高突变频率均最高,分别为2.833％、3.473％和1.828％,相互作用系数分别为1.649、1.329和1.449。

MUTAGENIC EFFECTS OF ~(137)Cs GAMMA RAY AND SODIUM AZIDE COMBINED TREATMENT ON RICE(Oryza sativa L. )

Dry seeds of rice variety,Guanglu No.4,were treated with 50,100,150,200,250,300Gy of gamma ray or 1,2,3 mmol of sodium azide (NaN3),and with their combination.The biological effects in MI and mutation frequency of M2 were investigated.The results showed that the biological injuries of Ml increased with dose of gamma ray and concentration of NaN3.The additive effects were observed on seedling height,root length,vigorous index of seedling height,vigorous index of root length and the synergic effect on the percentage of chromosome aberration.It was found that combination treatment with gamma ray and sodium azide were more effective than single treatment of gamma ray or NaN3.The results of this study suggested that 200Gy gamma ray +2mmol NaN3 was the most effective one for inducing mutation.Frequency of chlorophyll mutation,mutation on heading date and the plant height in M2 generation were 2.833%,2.473% and 1.828% respectively.Index of interaction were 1.640,1.329 and 1.449 respectively.