辐射技术制备固定化蛋白酶

本文探讨了在低温条件下,~(60)Co-γ射线诱导亲水性单体丙烯酸羟乙酯、疏水性的聚乙二醇二甲基丙烯酸酯系列单体聚合固定化蛋白酶的方法,研究了单体的分子结构,单体性质对固定化蛋白酶活性和热稳定性的影响。实验结果表明,采用单官能团单体(HEA)制备的固定化蛋白酶相对活性高于双官能团单体,固定化酶相对活性随疏水性单体聚乙二醇二甲基丙烯酸酯中乙二醇数的增加而增加。对于亲水性单体HEA固定化蛋白酶的相对活性则随单体的冷却速率的增加而增加。固定化蛋白酶的热稳定性随单体的亲水性而变化。

IMMOBILIZATION OF PROTEINASE BY RADIATION TECHNIQUE

A immobilization method of proteinase (Bacillus subtilis) Type Ⅷ by radiation-induced polymerization of hydrophilic monomer (2-hydroxyethyl acrylate,HEA) and a series of hy-drophobic monomers (polyethylene glycol dimethacrylate) at low temperature is introduced.The effect of monomer molecular structure and the relative activity of immobilized proteinase are also discussed.The resultshowed that the relative activity of immobilized enzyme prepared from monofunctional monomers was higher than that from bifunctional monomers and the activity increased with an increase in the number of glycol units of polyethlene glycol dimethacrylate monomers,and it also increased with the cooling rate of hydrophilic monomers (HEA).The thermal stability of the immobilized enzymes varied with hydrophili-ty of the monomers.