| ‘黄河蜜3号’高效再生体系的建立 |
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| 引用本文： | 毛娟,陈大鹏,赵长增.‘黄河蜜3号’高效再生体系的建立[J].甘肃农业大学学报,2010,45(2). |
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| 作者姓名： | 毛娟 陈大鹏 赵长增 |
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| 作者单位： | 1. 甘肃农业大学农学院,甘肃,兰州,730070
2. 甘肃省农业科学院农产品贮藏加工研究所,甘肃,兰州,730070 |
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| 基金项目： | 甘肃省农牧厅生物技术专项(GNSW-2004-04) |
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| 摘 要： | 以厚皮甜瓜‘黄河蜜3号’近轴端、远轴端子叶以及下胚轴作为外植体,在多种激素配比组合的培养基上进行分化诱导不定芽试验,并对发生的不定芽伸长和无根苗发根进行培养基的筛选.结果表明:适合‘黄河蜜’不定芽诱导的外植体材料为近轴端子叶,培养基激素组合为MS+2.0 mg.L-16-BA+0.1 mg.L-1IAA;伸长培养基激素组合为MS+0.1 mg.L-16-BA+0.1 mg.L-1IAA;生根培养基为1/3MS.
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| 关 键 词： | ‘黄河蜜3号’ 不定芽 再生 |
Establishment of efficient regeneration system of Cucumis melo cv. 'Huanghemi No.3' |
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| MAO Juan,CHEN Da-peng,ZHAO Chang-zeng.Establishment of efficient regeneration system of Cucumis melo cv. 'Huanghemi No.3'[J].Journal of Gansu Agricultural University,2010,45(2). |
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| Authors: | MAO Juan CHEN Da-peng ZHAO Chang-zeng |
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| Institution: | 1.College of Agronomy;Gansu Agricultural University;Lanzhou 730070;China;2.Agricultural Product Storage and Processing Research Institute;Gansu Academy of Agricultural Sciences;China |
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| Abstract: | The regeneration ability of Cucumis melo L. cv. 'Huanghemi No.3' was studied.Three different explants,proximal cotyledon,adaxial cotyledon and hypocotyl,were cultured in mediums of different hormone combination to induce adventitious bud,and the best elongation and rooting medium were choose.The results showed that the best explant for adventitious bud inducing was proximal cotyledon,the optimum hormone combination was MS+2.0 mg·L-16-BA+0.1 mg·L-1IAA,the optimum bud elongating medium was MS+0.1 mg·L-16-BA+0.1 mg·L-1IAA,and the best in vitro rooting medium was 1/3MS. |
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| Keywords: | Cucumis melo L cv 'Huanghemi No 3' adventitious bud regeneration |
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