水稻颖壳退化突变体degenerated hull 3 (dh3)的表型分析与基因定位

水稻(Oryza sativaL.)花器官的发育直接影响其产量和品质。本研究报道了一个水稻颖壳退化突变体，来源于恢复系缙恢10号的ethyl methane sulfonate (EMS)诱变群体，命名为degenerated hull 3 (dh3)。该突变体表现为内外稃退化变窄，且不能正常闭合。在一些突变严重的小花中，外稃甚至退化成芒状，内稃边缘和浆片退化变窄且融合。遗传分析表明该性状受1对隐性基因调控。利用不育系西农1A与dh3杂交构建的356株F₂突变群体，将DH3基因精细定位在第12染色体的SSR标记RM27706和RM27709之间，物理距离为44.72 kb，该区段内未见已知功能基因的报道。本研究的结果为以后DH3基因的图位克隆与功能分析打下基础。

Phenotypic Analysis and Gene Mapping of degenerated hull 3 (dh3) Mutant in Rice (Oryza sativa L.)

The floral organ development directly influences the yield and quality of rice. In this study, we reported a mutant degenerated hull 3 (dh3), derived from ethylmethane sulfonate (EMS)-treated Jinhui10 (Oryza sativa L. ssp. indica). The manifestation of dh3 mutant was degradation and narrowing in lemma and palea, losting the ability of close-up. In some seriously mutated florets, the lemma even degenerated in an awn-like shape, the margin region of palea and lodicules became narrow, even fused. Genetic analysis indicated that the dh3 character is controlled by a recessive gene. By using 356 F₂ mutants derived from a cross between sterile line Xinong 1A and dh3, DH3 was mapped between SSR markers RM27706 and RM27709 on chromosome 12 in rice, with a physical distance of 44.72 kb. There is no report of known functional gene in this zone. The results will shed light on the future clone and function analysis of DH3 gene.