Comparison of isozyme and random amplified polymorphic DNA data for determining intraspecific variation in Cucumis |
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Authors: | Jack E Staub Jodie Box Vladimir Meglic Thomas F Horejsi JD McCreight |
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Institution: | (1) USDA-ARS, Vegetable Crops Unit, Department of Horticulture, University of Wisconsin, 1575 Linden Dr., Madison, WI 53706, USA;(2) USDA-ARS, Crop Improvement and Protection Unit, 1636 East Alisal Street, Salinas, CA, 93905 |
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Abstract: | Variation at isozyme and random amplified polymorphic DNA (RAPD) loci in eight cucumber and seven melon cultivars, breeding lines, and plant introductions were used to determine the utility of these markers for assessing genetic variation among populations of each species. Although dendrograms derived from cluster analyses using species' variation at marker loci were dissimilar, these disparities were consistent with differences in the pedigrees and/or other information (e.g., morphological) known about each accession and species. Empirical estimations of variances associated with each marker type in the cucumber and melon accessions examined indicate that, per band, lower coefficients of variation can be attained in the estimation of genetic difference when using RAPDs compared to isozymes. The disparity between the marker analyses made may be related to the amount of genome coverage characteristic of a particular marker system in a species and its efficiency in sampling variation in a population. |
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Keywords: | African horned cucumber cluster analysis cucumber Cucumis melo Cucumis metuliferus Cucumis sativus genetic distance genetic markers isozymes melon muskmelon RAPDs |
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