鸡IL-2重组杆状病毒的构建及其病理组织学研究

以鸡白细胞介素2(IL-2)为免疫刺激因子,探讨鸡IL-2重组杆状病毒(BV-LMI-IL-2)对鸡脏器的影响,为IL-2促进疫苗免疫的研究提供理论依据。以IL-2为目的基因,以p LMI为杆状病毒转移载体,构建BV-LMI-IL-2。最终使用BV-LMI-IL-2与新城疫(NDV)抗原疫苗BV-LMI-F进行单独免疫和联合免疫,观察鸡腺胃、肝脏、心脏、十二指肠等器官病理变化情况。免疫和攻毒试验结果显示:BV-LMI-F与BV-LMI-IL-2联合免疫时,保护率提高了12.5%,说明BV-LMI-IL-2对疫苗起到了免疫增强作用,可以作为免疫增强剂使用。对攻毒后的对照组死亡鸡只和免疫组死亡的鸡只剖检,观察病变器官和病理组织切片结果显示,联合免疫组器官无明显病理变化,证明鸡IL-2重组杆状病毒提高了对鸡脏器器官的免疫保护作用。

Construction of Chicken IL-2 Recombinant Baculovirus and Its Histopathological Study

To investigate the effect of chicken interleukin-2 (IL-2) recombinant baculovirus (BV-LMI-IL-2) on chicken visceral organs and provide scientific basis for developing newcastle disease (ND) vaccines, IL-2 was taken as the immune stimulating factor. The gene encoding IL-2 was inserted to the baculovirus transfer vector pLMI, thus BV-LMI-IL-2 was obtained. Finally BV-LMI-IL-2 and ND antigen vaccine BV-LMI-F were used to conduct independent and combined immunization. At the same time, pathological changes in organs including glandular stomach, liver, heart and duodenum were observed. Immune and challenge experiment showed that the protection rate of combined immunization of BV-LMI-IL- 2 with BV-LMI-F increased by 12.5%. This indicated that BV-LMI-IL-2 could enhance the immune function and be used as an immune enhancer. The chicken in the control group and immune group were dissected. Observation of diseased organs and histopathologic slide showed that organs in combined immunization group were without obvious pathological changes. It was proved that the chicken IL- 2 recombinant baculovirus improved the immune protective effect on chicken organs.