马立克氏病病毒MEQ蛋白对MDV增殖影响的研究

在马立克氏病病毒MDV致病机理的研究中，弄清致病基因meq与病毒增殖之间的关系及其分子机制是十分重要的基础，以重组反转录病毒（RCAS-meq)感染鸡胚成纤维细胞（CEF），使源于MDV强毒参考株（vMDV)GA株的meq基因表达于宿主细胞内，然后再用GA株感染这些细胞。通过利用MDV强毒株特异的抗pp38单克隆抗体所进行的“黑斑”试验以确定MDV的增殖水平，并与未接种RCAS-meq的CEF进行比较。研究结果发现，细胞内表达的meq基国产物可促进GA株于体外培养细胞中的感染与增殖（病毒斑数增多）。根据试验的结果，作者认为meq基因在感染细胞内的表达水平是MDV增殖以及进而能致病、致瘤的分子基础。

The evaluation of the effect og meq protein on the replication of marek's disease virus in vitro

In the study of pathogenicity and oncogenicity of Marek's disease virus (MDV), the revelation of the relation and the mechanism between the activity of oncogene meq and virus replication in molecular level is an important foundation. Monolayers of chicken embryo fibroblast (CEF) were infected with a recombinant retrovirus (RCASmeq), which carrying and expressing meq gene originated from virulent MDV (vMDV) reference strain GA, and then reinfected with GA 12 hours postinfecton (PI) of RCAS meq . Black plaque assay (BPA) was used to evaluate the replication of MDV and compared with those of the noninfected control. The experiment showed that the expression of meq gene within the cells could enhance the infection and replication of GA in vitro by the increase of virus plaque number. The results demonstrated that the expression activity of the meq gene is the molecular basis for the replication and the subsequent pathogenicity and oncogenicity of vMDV.