| 大豆硫氧还蛋白基因的克隆与分析 |
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| 引用本文： | 王伟旗,侯文胜.大豆硫氧还蛋白基因的克隆与分析[J].大豆科学,2011,30(3):351-355. |
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| 作者姓名： | 王伟旗 侯文胜 |
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| 作者单位： | 中国农业科学院作物科学研究所国家农作物基因资源与基因改良重大科学工程; |
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| 基金项目： | 国家自然科学基金资助项目(30771358) |
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| 摘 要： | 通过对大豆耐盐品种文丰7盐处理抑制差减文库的筛选,获得了一些差异表
达的EST序列,与NCBI中EST数据库进行比对分析后发现,其中1个与硫氧还蛋白相关.据此预测了大豆硫氧还蛋白(Thioredoxin,Trx)基因的cDNA序列,并采用RT-PCR方法克隆了大豆Trx基因.生物信息学分析表明:该基因包含1个354 b...
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| 关 键 词： | 大豆 硫氧还蛋白基因 基因克隆 |
Clone and Analysis of Thioredoxin Gene from Soybean(Glycine max L.) |
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| WANG Wei-qi,HOU Wen-sheng.Clone and Analysis of Thioredoxin Gene from Soybean(Glycine max L.)[J].Soybean Science,2011,30(3):351-355. |
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| Authors: | WANG Wei-qi HOU Wen-sheng |
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| Institution: | WANG Wei-qi,HOU Wen-sheng(National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI),Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China) |
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| Abstract: | Based on screening the suppression subtractive hybridization library which constructed by soybean cultivar 'wenfeng7'(salt-tolerant) after salt treatment,we obtained some differentially expressed EST sequences.The EST sequences were compared with the NCBI databases,analysis result showed that one EST sequence related to thioredoxin.A novel thioredoxin gene(Trx) of Glycine max was cloned and identified with the EST obtained from suppression subtractive hybridization library.Bioinformatics analysis showed tha... |
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| Keywords: | Soybean Salt Thioredoxin gene Gene clone |
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