| 不同基因型大豆Fd-GOGAT基因cDNA序列的克隆与分析 |
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| 引用本文： | 陈丽华,刘丽君,刘页丽,祖伟. 不同基因型大豆Fd-GOGAT基因cDNA序列的克隆与分析[J]. 大豆科学, 2011, 30(3): 374-378 |
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| 作者姓名： | 陈丽华 刘丽君 刘页丽 祖伟 |
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| 作者单位： | 国家农业标准化监测与研究中心;东北农业大学农学院; |
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| 基金项目： | 国家“十一五”科技攻关资助项目(2006BAD21B01) |
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| 摘 要： | 克隆了6个不同基因型大豆的Fd-GOGAT基因,序列比对分析结果表明:不同基因型大豆的Fd-GOGAT基因序列相似性很高,可设计出通用Real-time PCR引物,检测Fd-GOGAT基因的表达规律.在东农42 Fd-GOGAT基因序列的编码区内出现了1个8核苷酸的缺失,产生了移码突变,导致其C端缺失了79个氨基酸的...
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| 关 键 词： | 大豆 Fd-GOGAT基因 克隆 |
Cloning and Analysis of Fd-GOGAT Genes from Different Soybean Varieties |
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| CHEN Li-hua,LIU Li-jun,LIU Ye-li,ZU Wei. Cloning and Analysis of Fd-GOGAT Genes from Different Soybean Varieties[J]. Soybean Science, 2011, 30(3): 374-378 |
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| Authors: | CHEN Li-hua LIU Li-jun LIU Ye-li ZU Wei |
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| Affiliation: | CHEN Li-hua1,LIU Li-jun2,LIU Ye-li2,ZU Wei2(1.National Agriculture Standardize Supervise and Research Center,Harbin 150036,2.Agronomy College,Northeast Agricultural University,Harbin 150030,Heilongjiang,China) |
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| Abstract: | Fd-GOGAT genes from six different soybean varieties were cloned.The comparative analysis showed the sequences of GOGAT genes in different soybean varieties were very identical,so we can design general real-time PCR primer to detect express regulation of Fd-GOGAT genes.The coding sequence of Fd-GOGAT gene in Dongnong42 appeared an absence of 8 nucleotides,which brought frameshift mutation,led to its C-terminal lack of 79 amimo acids conserved sequence and the GS1 big subunit C-terminal conserved domain(gltB_... |
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| Keywords: | Soybean Fd-GOGAT genes Enrichment separation Clone |
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