| 诱导大豆抗阿特拉津青霉snef960菌株摇瓶发酵条件的筛选 |
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| 引用本文： | 潘琳琳,段玉玺,陈立杰,姜美英,刘大伟,潘阳,李颂.诱导大豆抗阿特拉津青霉snef960菌株摇瓶发酵条件的筛选[J].大豆科学,2011,30(2):272-276. |
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| 作者姓名： | 潘琳琳 段玉玺 陈立杰 姜美英 刘大伟 潘阳 李颂 |
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| 作者单位： | 沈阳农业大学植物保护学院; |
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| 基金项目： | 现代农业产业技术体系岗位科学家专项资助项目 |
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| 摘 要： | 从实验室保存菌种中筛选出一株具有诱导大豆抗阿特拉津的青霉菌株snef960.利用正交试验优化了发酵培养基组分,其最佳碳氮比为葡萄糖(4%)、硝酸铵(0.6%),发酵培养基中4种无机盐的最佳配比为:氯化钾(0.04%)、磷酸氢二钾(0.15%)、硫酸镁(0.04%)、硫酸亚铁(0.001%).通过单因素试验确定青霉sne...
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| 关 键 词： | 大豆 阿特拉津 真菌 筛选 发酵条件 |
Screening of Fermentation Conditions for Fungi snef960 to Induce Anti-atrazine in Soybean |
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| PAN Lin-lin,DUAN Yu-xi,CHEN Li-jie,JIANG Mei-ying,LIU Da-wei,PAN Yang,LI Song.Screening of Fermentation Conditions for Fungi snef960 to Induce Anti-atrazine in Soybean[J].Soybean Science,2011,30(2):272-276. |
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| Authors: | PAN Lin-lin DUAN Yu-xi CHEN Li-jie JIANG Mei-ying LIU Da-wei PAN Yang LI Song |
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| Institution: | PAN Lin-lin,DUAN Yu-xi,CHEN Li-jie,JIANG Mei-ying,LIU Da-wei,PAN Yang,LI Song(College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,Liaoning,China) |
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| Abstract: | Snef 960 which have efficiency to induce anti-atrazine in soybean was screened from laboratory save strains.Cultivation medium of snef960 was researched by orthogonal experiment.The result showed that the optimal C/N ratio was composed of 4% glucose,0.6% NH4NO3,the optimal inorganic salts ratio was composed of 0.04% KCl,0.15% K2HPO4,0.04% MgSO4·7H2O,0.001% FeSO4.The culture conditions were screened with mono-factor test,and the most suitable technical parameters were summed up as follows: pH8 before sterilized,fementation time 9 d,agitation 180 r·min-1,volume 70/100mL,inoculated volume 10%. |
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| Keywords: | Soybean Atrazine Fungi Screening Fermentation conditions |
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