| 大豆子叶节植株再生体系的研究 |
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| 引用本文： | 赖冰冰,韩阳,李春风,张兴政,张立军,宋书宏. 大豆子叶节植株再生体系的研究[J]. 大豆科学, 2011, 30(2): 303-305 |
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| 作者姓名： | 赖冰冰 韩阳 李春风 张兴政 张立军 宋书宏 |
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| 作者单位： | 辽宁大学,生命科学院,辽宁,沈阳,110036;辽宁大学,生命科学院,辽宁,沈阳,110036;沈阳农业大学,生物科学技术学院,辽宁,沈阳,110866;辽宁省农业科学院作物所,辽宁,沈阳,110866;沈阳农业大学,生物科学技术学院,辽宁,沈阳,110866;辽宁省农业科学院作物所,辽宁,沈阳,110866 |
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| 基金项目： | 国家科技攻关资助项目(2003ba538c) |
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| 摘 要： | 以大豆品种辽豆17、辽豆18和辽豆23子叶节为外植体诱导不定芽的发生.结果表明:随着培养基中6-BA浓度的增加,每块外植体上不定芽的数量呈增加的趋势,当向1/2MSB培养基中添加0.05 mg·L-1IBA和4.0 mg·L-16.BA时,不定芽的诱导率最高.比较不同的外植体获得方法,保留半片子叶的外植体诱...
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| 关 键 词： | 大豆 子叶节 不定芽 植株再生 |
Research on Cotyledonary Nodes Regeneration System of Soybean |
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| LAI Bing-bing,HAN Yang,LI Chun-feng,ZHANG Xing-zheng,ZHANG Li-jun,SONG Shu-hong. Research on Cotyledonary Nodes Regeneration System of Soybean[J]. Soybean Science, 2011, 30(2): 303-305 |
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| Authors: | LAI Bing-bing HAN Yang LI Chun-feng ZHANG Xing-zheng ZHANG Li-jun SONG Shu-hong |
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| Affiliation: | LAI Bing-bing1,HAN Yang1,2,3,LI Chun-feng1,ZHANG Xing-zheng1,ZHANG Li-jun2,SONG Shu-hong3(1.School of Life Science,Liaoning University,Shenyang 110036,2.College of Biological Sciences and Technology,Shenyang Agricultural University,Shenyang 110866,3.Crop Institute,Liaoning Academy of Agricultural Science,Liaoning,China) |
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| Abstract: | The adventive shoots were induced from cotyledon nodes with soybean cultivar Liaodou 17,Liaodou 18 and Liaodou 23 as explants.With the increase of 6-BA concentration,the number of adventitious shoots on each explant showed an increasing trend,when 0.05 mg·L-1 IBA and 4.0 mg·L-1 6-BA were added into 1/2MSB medium,the induction rate of adventive shoots is the highest.Compared the effect of explants,the explants of reserving half cotyledon was better than the cotyledon nodes without cotyledon.Therefore,the optimum culture medium for elongation of adventitious bud was 1/2MSB+0.5 mg·L-1 6-BA+0.05 mg·L-1 IBA,and the optimum culture medium for rooting was 1/2MSB+1.0 mg·L-1 IBA. |
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| Keywords: | Soybean Cotyledonary node Adventive shoots Plant Regeneration |
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