| 植酸酶基因phyA转化大豆品种研究 |
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| 引用本文： | 闫瑞叶,李喜焕,李桂兰,常文锁,张彩英.植酸酶基因phyA转化大豆品种研究[J].大豆科学,2011,30(3):356-361. |
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| 作者姓名： | 闫瑞叶 李喜焕 李桂兰 常文锁 张彩英 |
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| 作者单位： | 河北农业大学教育部华北作物种质资源研究与利用重点实验室;河北科技师范学院生命科技学院; |
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| 基金项目： | 国家转基因重大专项资助项目(2009ZX08004-004B); 国家自然科学基金资助项目(31071441); 河北省自然科学基金资助项目(C2010000749); 河北省教育厅科学研究资助项目(2009) |
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| 摘 要： | 土壤磷多以有机态形式存在,而植酸磷占一半以上,分解利用植酸态磷是提高土壤磷利用效率、改善植物磷素营养的新途径.利用大豆农杆菌介导子叶节转化与花粉管通道转化技术,将含有根特异启动子pyk10、信号肽S和植酸酶phyA的嵌和基因(KSA)转入冀豆12、冀豆16、五星1号和吉林35中.经PCR检测,共获得T0阳性植株114个...
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| 关 键 词： | 大豆 植酸酶基因 遗传转化 农杆菌介导 |
Transgenic Soybean(Glycine max.L) Plants with phyA Gene Improved Phytase Activity |
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| YAN Rui-ye,LI Xi-huan,LI Gui-lan,CHANG Wen-suo,ZHANG Cai-ying.Transgenic Soybean(Glycine max.L) Plants with phyA Gene Improved Phytase Activity[J].Soybean Science,2011,30(3):356-361. |
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| Authors: | YAN Rui-ye LI Xi-huan LI Gui-lan CHANG Wen-suo ZHANG Cai-ying |
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| Institution: | YAN Rui-ye1,LI Xi-huan1,LI Gui-lan2,CHANG Wen-suo1,ZHANG Cai-ying1(1.North China Key Laboratory of Crop Germplasm Resources,Education Ministry of China,Agricultural University of Hebei,Baoding 071001,2.Life Science and Technology College,Hebei Normal University of Science & Technology,Changli 066600,Hebei,China) |
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| Abstract: | Organic phosphorus(P) is the main form of phosphorus in soil,of which the phytate accounted for more than 50%.The available way that improves plant P nutrition is to hydrolyze phytate into inorganic phosphorus(Pi) to increase the soil P utilization efficiency.In this study,a phytase gene(phyA),driven by the root-specific pyk10 promoter and directed by a carrot extracellular targeting peptide,was introduced into different soybean varieties(Jidou 12,Jidou 16,Wuxing 1 and Jilin 35) by Agrobacterium-mediated an... |
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| Keywords: | Soybean Phytase gene Genetic transformation Agrobacterium-mediated |
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