| 新聚丙烯酰胺凝胶电泳快速检测大豆脂氧酶缺失方法 |
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| 引用本文： | 王绍东,姜妍,王浩,刘伟,李远明,李宏伟,李文滨. 新聚丙烯酰胺凝胶电泳快速检测大豆脂氧酶缺失方法[J]. 大豆科学, 2011, 30(3): 484-487 |
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| 作者姓名： | 王绍东 姜妍 王浩 刘伟 李远明 李宏伟 李文滨 |
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| 作者单位： | 东北农业大学国家大豆工程技术研究中心,黑龙江哈尔滨,150030;东北农业大学大豆生物学教育部重点实验室,黑龙江哈尔滨,150030 |
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| 基金项目： | 黑龙江省留学回国基金资助项目(LC2009C25); 哈尔滨市科技创新人才研究专项资金资助项目(2010RFLYN016) |
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| 摘 要： | 通过对传统SDS-PAGE电泳技术中样品前处理及制胶技术的改进,开发出了一种能够清晰鉴别Lox-1与Lox-2同工酶的SDS-PAGE电泳快速检测新技术.利用新改进的方法,在289个CS8000(9)×FJ307(♂)F2杂交后代中,选拔出Lox-1,-2,-3同工酶完全缺失的个体27株,以及Lox-1,-3和Lox-...
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| 关 键 词： | 脂氧酶缺失 聚丙烯酰胺凝胶电泳 杂交后代的筛选 大豆 |
A Fast Detection Method for Screening of Lipoxygenase Null Individual Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis in Soybean |
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| WANG Shao-dong,JIANG Yan,WANG Hao,LIU Wei,LI Yuan-ming,LI Hong-wei,LI Wen-bin. A Fast Detection Method for Screening of Lipoxygenase Null Individual Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis in Soybean[J]. Soybean Science, 2011, 30(3): 484-487 |
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| Authors: | WANG Shao-dong JIANG Yan WANG Hao LIU Wei LI Yuan-ming LI Hong-wei LI Wen-bin |
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| Affiliation: | WANG Shao-dong1,JIANG Yan1,WANG Hao1,LIU Wei1,LI Yuan-ming1,LI Hong-wei1,LI Wen-bin2(1.National Research Center of Soybean Engineering and Technology(NRCSET),Northeast Agricultural University,Harbin 150030,2.Key Laboratory of Soybean Biology of Chinese Education Ministry,Heilongjiang,China) |
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| Abstract: | In this research,through the improvement of the sample pretreatment and confection of the gel,a fast detection method were developed for screening of lipoxygenase(Lox) null individual using sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) in soybean.By this method,we have screened 27 strains of the Lox-1,-2,-3 null,and some Lox-1,-3 or Lox-2,-3 incompletion lacking individuals from 289 individuals in the F2(CS8000(♀)×FJ307(♂))generations.The results proved that the method was better in precision,velocity and less costs of the screening.It is significant for selecting the lipoxygenase null individual in quality improvement breeding of soybean. |
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| Keywords: | Lipoxygenase null Sodium dodecyl sulfate polyacrylamide gel electrophoresis Screening of filial generation Soybean |
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