The outer membrane protein P2 (OmpP2) of Haemophilus parasuis induces proinflammatory cytokine mRNA expression in porcine alveolar macrophages |
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| Authors: | Suming Zhou Xianhui He Chenggang Xu Bing Zhang Saixiang Feng Yi Zou Jingyi Li Ming Liao |
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| Affiliation: | 1. Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, People’s Republic of China;2. Key Laboratory of Applied Marine Biotechnology, School of Marine Science, Ningbo University, Ningbo 315211, People’s Republic of China;3. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, People’s Republic of China;1. Institute of Solid State Electronics, Technische Universität Dresden, 01062 Dresden, Germany;2. Department of Paediatrics, University Hospital Carl Gustav Carus, Fetscherstr. 74, 01307 Dresden, Germany;1. Department of Basic Medical Sciences, College of Medicine, QU Health Cluster, Qatar University, Doha, Qatar;2. Department of Microbiology, Faculty of Medicine, Kuwait University, Jabriya, Kuwait;1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, China;2. College of Veterinery Medicine, Huazhong Agricultural University, China;3. Hubei Provincial Key Laboratory of Preventive Veterinary Medicine, Huazhong Agricultural University, China;4. Hubei Animal Disease Prevention and Control Center, Wuhan, China |
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| Abstract: | Porins, expressed by Gram-negative bacteria, have several biological effects on host tissues or cells. The outer membrane protein P2 (OmpP2), a member of the porin family, has been identified as a multifunctional protein involved in the pathogenicity of Haemophilus parasuis. In the present study, it was shown that OmpP2 (0.5–10 μg/mL) from H. parasuis Nagasaki strain up-regulated mRNA expression of interleukin (IL)-1α, IL-1β, IL-6 and IL-8 in porcine alveolar macrophages (PAM, 3D4/31) in vitro, in a dose-dependent manner. Moreover, OmpP2 porin induced a more prolonged cytokine response in PAM than that of the lipooligosaccharide (LOS) from this microorganism. The data demonstrate that H. parasuis OmpP2 can stimulate proinflammatory cytokine mRNA expression, suggesting that this particular porin might play an important role in the pathogenesis of disease caused by H. parasuis. |
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| Keywords: | OmpP2 porin Alveolar macrophage Proinflammatory cytokines |
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