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Stimulation of airway neutrophils following dexamethasone administration and equid herpesvirus-2 challenge in horses
Authors:Eric A Richard  Pierre-Hugues Pitel  Laurent Lemaitre  Dominique Jas  Pierre Lekeux  Stéphane Pronost  Guillaume Fortier
Institution:1. Normandie Université, UNICAEN, SF 4206 ICORE, Frank Duncombe Laboratory, 1 Route de Rosel, 14053 Caen cedex 4, France;2. Merial S.A.S., 29 Avenue Tony Garnier, 69007 Lyon, France;3. Department of Physiology, Faculty of Veterinary Medicine, University of Liège, 20 Boulevard de Colonster, Bat. B42, 4000 Liège, Belgium;4. Normandie Université, UNICAEN, EA 4655 – U2RM, 14032 Caen, France;1. Department of Veterinary Science and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy;2. Veterinary Laboratory, Poggio dei Pini, 09012 Capoterra, Cagliari, Italy;3. Clinical Pathology Unit, Central Laboratory, Veterinary Teaching Hospital, University of Milan, Via dell’Università 6, 26900 Lodi, Italy;1. Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, 625 Harrison St., West Lafayette, IN 47907, USA;2. Department of Comparative Pathobiology, Purdue University, 625 Harrison St., West Lafayette, IN 47907, USA;1. Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA 95616, USA;2. Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA;3. Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA;1. Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland;2. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland;3. Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Tsushima Naka 1-1-1, Okayama 700-8530, Japan;1. School of Veterinary Medicine and Animal Science, Universidade Estadual Paulista, Department of Veterinary Clinical Science, Botucatu, São Paulo, Brazil;2. College of Veterinary Medicine, Cornell University, Department of Molecular Medicine, Ithaca, NY, USA
Abstract:The aim of this study was to investigate neutrophil stimulation following experimentally-induced airway inflammation in healthy horses. Six horses received dexamethasone and four were then inoculated with equid herpesvirus-2 (EHV-2). Significant neutrophilia was detected in tracheal wash and bronchoalveolar lavage fluid for up to 6 days. Concentrations of neutrophil elastase (NE) and myeloperoxidase (MPO) were significantly increased compared to baseline for up to 14 days in tracheal washes and both markers were significantly correlated with neutrophil counts. Serum levels of surfactant protein D were not significantly modified throughout the study. These results suggest that dexamethasone administration with or without EHV-2 inoculation is associated with a sustainable activation and degranulation of neutrophils in the trachea along with moderate modifications detectable in the lower airways.
Keywords:Neutrophil elastase  Myeloperoxidase  Corticosteroids  Airway inflammation  Horse
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