Culture of canine synoviocytes on porcine intestinal submucosa scaffolds as a strategy for meniscal tissue engineering for treatment of meniscal injury in dogs |
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| Authors: | Jennifer J Warnock Jason Spina Gerd Bobe Katja F Duesterdieck-Zellmer Jesse Ott Wendy I Baltzer Brian K Bay |
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| Institution: | 1. Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA;2. Department of Animal and Rangeland Sciences, Oregon State University, Corvallis, OR 97331, USA;3. Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA;4. School of Mechanical, Industrial, and Manufacturing Engineering, Oregon State University, Corvallis, OR 97331, USA;1. Comparative Pain Research Laboratory (CPRL), Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA;2. Center for Comparative Medicine and Translational Research, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA;3. Department of Statistics, North Carolina State University, Raleigh, NC 27695, USA;4. Department of Clinical Sciences Surgery Section, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA;1. Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, College of Bioresource Science, Nihon University, Fujisawa 252-0880, Japan;2. Department of Functional Morphology, Division of Cell Regeneration and Transplantation, Nihon University School of Medicine, Tokyo 173-8610, Japan;3. Laboratory of Cell and Tissue Biology, College of Bioresource Science, Nihon University, Fujisawa 252-0880, Japan;1. Scitech Associates, LLC, 232 Woodland Drive, State College, PA 16803, USA;2. Department of Physics, 104 Davey Laboratory, The Pennsylvania State University, University Park, PA 16802, USA;3. Facultés Universitaires Notre-Dame de la Paix, Rue de Bruxelles 61, Namur 5000, Belgium;4. Department of Physics, University of Ulsan, Ulsan 680-749, Republic of Korea |
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| Abstract: | Meniscal injury is a common cause of canine lameness. Tissue engineered bioscaffolds may be a treatment option for dogs suffering from meniscal damage. The aim of this study was to compare in vitro meniscal-like matrix formation and biomechanical properties of porcine intestinal submucosa sheets (SIS), used in canine meniscal regenerative medicine, to synoviocyte-seeded SIS bioscaffold (SSB), cultured with fetal bovine serum (SSBfbs) or chondrogenic growth factors (SSBgf). Synoviocytes from nine dogs were seeded on SIS and cultured for 30 days with 17.7% fetal bovine serum or recombinant chondrogenic growth factors (IGF-1, TGFβ1 and bFGF). The effect on fibrochondrogenesis was determined by comparing mRNA expression of collagen types Iα and IIα, aggrecan, and Sry-type homeobox protein-9 (SOX9) as well as protein expression of collagens I and II, glycosaminoglycan (GAG), and hydroxyproline.The effect of synoviocyte seeding and culture conditions on biochemical properties was determined by measuring peak load, tensile stiffness, resilience, and toughness of bioscaffolds. Pre-culture SIS contained 13.6% collagen and 2.9% double-stranded DNA. Chondrogenic growth factor treatment significantly increased SOX9, collagens I and IIα, aggrecan gene expression (P < 0.05), and histological deposition of fibrocartilage extracellular matrix (GAG and collagen II). Culture with synoviocytes increased SIS tensile peak load at failure, resilience, and toughness of bioscaffolds (P < 0.05). In conclusion, culturing SIS with synoviocytes prior to implantation might provide biomechanical benefits, and chondrogenic growth factor treatment of cultured synoviocytes improves in vitro axial meniscal matrix formation. |
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| Keywords: | Tissue engineering Meniscus Synovium Cell culture Scaffolds |
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