桑叶提取物和1-脱氧野尻霉素对大鲵生长性能、肝脏功能及免疫能力的影响

本试验旨在研究桑叶提取物(MLE)和1-脱氧野尻霉素(DNJ)对大鲵生长性能、肝脏功能及免疫能力的影响.选择初始均重为(47.81±0.23)g的大鲵72尾,随机分为3组,每组3个重复,每个重复8尾.对照组饲喂基础饲料,试验组在基础饲料中分别添加7.5 g/kg MLE(MLE组)和0.04 g/kg DNJ(DNJ组...

Effects of Mulberry Leaf Extract and 1-Deoxynojirimycin on Growth Performance,Liver Function and Immune Ability of Giant Salamander(Andrias davidianus)

This experiment was conducted to explore the effects of mulberry leaf extract(MLE)and 1-deoxynojirimycin(DNJ)on growth performance,liver function and immune ability of giant salamander(Andrias davidianus).Seventy-two Andrias davidianus with initial average weight of(47.81±0.23)g were randomly divided into 3 groups with 3 replicates per group and 8 fish per replicate.The control group were fed a basal diet,and the experimental groups were fed basal diets supplemented with 7.5 g/kg MLE(MLE group)and 0.04 g/kg DNJ(DNJ group),respectively.The experiment lasted for 90 days.The results showed as follows:1)the final body weight,weight gain rate(WGR),specific growth rate(SGR)and feed intake(FI)of MLE and DNJ groups were significantly higher than those of the control group(P<0.05),and the feed conversion rates(FCR)was significantly lower than that of the control group(P<0.05).2)The contents of ether extract in muscle and liver of MLE and DNJ groups were significantly lower than those of the control group(P<0.05).The muscle crude protein content of MLE and DNJ groups was significantly higher than that of the control group(P<0.05).3)The activities of carnitine palmitoyltransferase 1(CPT-1)and acetyl CoA carboxylase(ACC)and CPT-1/ACC in plasma of MLE and DNJ groups were significantly higher than those of the control group(P<0.05),and the DNJ group was significantly higher than MLE group(P<0.05).The activities of aspartate aminotransferase(AST),alanine aminotransferase(ALT)in plasma of MLE and DNJ groups were significantly lower than those of the control group(P<0.05),and the MLE group was significantly lower than DNJ group(P<0.05).The total antioxidant capacity(T-AOC),total superoxide dismutase(T-SOD)activity in liver of MLE and DNJ groups were significantly higher than those of the control group(P<0.05),and the MLE group was significantly higher than DNJ group(P<0.05);the liver malondialdehyde(MDA)content of MLE and DNJ groups was significantly lower than that of the control group(P<0.05),and the MLE group was significantly lower than DNJ group(P<0.05).4)The plasma immunoglobulin M(IgM)content of MLE and DNJ groups was significantly higher than that of the control group(P<0.05),and the MLE group was significantly higher than DNJ group(P<0.05).The plasma reactive oxygen species(ROS)content of MLE and DNJ groups was significantly lower than that of the control group(P<0.05),and the DNJ group was significantly lower than MLE group(P<0.05).It is concluded that dietary addition of MLE and DNJ can improve the growth performance,liver function and immune ability of Andrias davidianus.MLE is better than DNJ on improving body composition and liver function,and DNJ is better than MLE on removal effect of ROS;the overall effect of MLE is better than DNJ.