|
|||||
|
|
| 6个地方鸡种Sox8基因编码区的PCR-SSCP分析 | |
| 引用本文： | 夏彦玲,刘娣,陈国宏.6个地方鸡种Sox8基因编码区的PCR-SSCP分析[J].东北农业大学学报,2008,39(6). |
| 作者姓名： | 夏彦玲 刘娣 陈国宏 |
| 作者单位： | 1. 东北林业大学野生动物资源学院,哈尔滨,150040 2. 黑龙江省农业科学院畜牧研究中心,哈尔滨,150086 3. 扬州大学动物科技学院,江苏,扬州,225009 |
| 摘 要： | 通过PCR-SSCP技术对6个地方鸡种的Sox8基因的编码区进行了单核苷酸多态性分析,在外显子3发现两个突变位点:即786 C→T和1 350 G→A。经统计分析和计算各种基因型频率及基因频率表明,在786 C→T的突变中,等位基因A在肉鸡中的频率比在蛋鸡中的频率高。因此,本试验得出趋势性结论:等位基因A的基因频率同脂肪性状呈正相关。 |
| 关 键 词： | 鸡育种 脂肪性状 Sox8 PCR-SSCP |
Polymorphism analysis of six native chicken breeds Sox8 cDNA by PCR-SSCP |
|
| XIA Yanling,LIU Di,CHEN Guohong.Polymorphism analysis of six native chicken breeds Sox8 cDNA by PCR-SSCP[J].Journal of Northeast Agricultural University,2008,39(6). | |
| Authors: | XIA Yanling LIU Di CHEN Guohong |
| Abstract: | PCR-SSCP technology was applied to detect SNPs of the exons of the Sox8 gene.The results showed that there were two mutant sites in the third exon(786 C→T and 1 350 G→A).Combining the computation of genotype frequency,gene frequency and statistical analysis with fat contain,this study found the frequency of allele A was higher in broilers than in layers and obtained a tendentious conclusion that the gene frequency of allele A was positively correlated with fat character. |
| Keywords: | Sox8 PCR-SSCP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |