微卫星标记OarHH35和BMS2508在4个山羊品种中的研究

选择与绵羊(Ovisaries)高繁殖力紧密关联的两个微卫星标记OarHH35和BMS2508,分析其在湘东黑山羊、南江黄羊、贵州黑山羊和波尔山羊中的多态性分布情况。结果表明,微卫星标记OarHH35在4个山羊(Caprahircus)品种中的等位基因数分别为11、8、7和10,BMS2508在4个山羊品种中的等位基因数分别为9、5、6和7,OarHH35/BMS2508在湘东黑山羊、南江黄羊、贵州黑山羊和波尔山羊中的多态信息含量值分别为0.8294/0.8047,0.8399/0.6894,0.7985/0.6910和0.8582/0.7688。2个微卫星基因座对湘东黑山羊产羔数的效应分析表明,微卫星标记BMS2508和OarHH35对山羊的产羔数有显著影响(P<0.05)。在OarHH35基因座,135/135bp基因型的最小二乘均值最高(3.67只/胎),123/135bp和125/125bp基因型的最小二乘均值也达到了3.00只/胎,表明等位基因135和125bp对湘东黑山羊产羔数具有显著的正效应。在BMS2508基因座,基因型132/145bp和93/132bp对产羔数效应的最小二乘均值分别为4.00和3.23只/胎,而基因型122/122bp对产羔数效应的最小二乘平均值只有1.00只/胎,因此等位基因145和93bp对湘东黑山羊产羔数有显著正效应,等位基因122bp对湘东黑山羊产羔数无显著效应。

Studies of Microsatellite Markers OarHH35 and BMS2508 in Four Goat Breeds

Two microsatellite markers OarHH35 and BMS2508 which were closely associated to the high reproduction in sheep (Ovis aries) were analyzed for polymorphisms in Xiangdong black goat, Nanjiang brown goat, Guizhou black goat and Boer goat. The number of alleles for OarHH35 was 11, 8, 7 and 10 respectively in four goat(capra hircus ) breeds. The number of alleles for BMS2508 was 9, 5, 6 and 7 respectively in four goat breeds. The polymorphic information contents for OarHH35/BMS2508 in Xiangdong black goat, Nanjiang brown goat, Guizhou black goat and Boer goat were 0.8294/0.8047,0.8399/0.6894, 0.7985/0.6910 and 0.8582/0.7688, respectively. It indicated that there were a significant influence (P < 0.05) of microsatellite markers BMS2508 and OarHH35 for litter size of goat of Xiangdong black goat. In the OarHH35 locus, the least squares means of genotype 135/135 bp was the highest (3.67 number born /litter ), thereinto the least squares means of genotype 123/135 bp and 125/125 bp also reached 3.00 number born /litter. It indicated that allele 135 and 125 bp for the litter size in Xiangdong black goat had significant positive effect. In the BMS2508 locus, the least squares means of genotype 132/145 and 93/132 bp for the litter size was 4.00 and 3.23 number born / litter respectively, but the least squares means of genotype 122/122 bp for the litter size only had 1.00 number born / litter. It indicated that allele 145 and 93 bp loci had significant positive effect on the litter size of Xiangdong black goat, and had significant negative effect between allele 122 bp and the litter size of Xiangdong black goat.