| 鸡致病性大肠杆菌肠毒素(LT)的鉴定及其基因的克隆 |
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| 引用本文： | 王文成,张贵刚,卫广森,李尚波,许崇波. 鸡致病性大肠杆菌肠毒素(LT)的鉴定及其基因的克隆[J]. 中国兽药杂志, 2002, 36(6): 26-28 |
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| 作者姓名： | 王文成 张贵刚 卫广森 李尚波 许崇波 |
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| 作者单位： | 1. 辽宁省益康生物物品厂,辽宁辽阳,111000
2. 宁夏大学生物工程系,宁夏银川,750021 |
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| 摘 要： | 鸡致病性大肠杆菌分离株O78经家兔肠袢结扎试验(RILT)证实,该菌株产生热敏性肠毒素(LT)。用PCR技术从该菌株中扩增出1.2kb的LT基因,然后将纯化的PCR产物克隆到pGEM-T载体中,转化至受体菌JM109中。用Amp/IPTG/X-gal琼脂平板蓝白菌落筛选得到阳性重组菌株,提取质粒用SphI和SalI双酶切鉴定,结果证实,构建的克隆质粒pXCLT1含有LT基因。
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| 关 键 词： | 大肠杆菌肠毒素 LT基因 基因克隆 |
| 文章编号： | 1002-1280(2002)06-0026-03 |
| 修稿时间： | 2002-03-12 |
Cloning of Heat-labile Enterotoxin Gene of Chicken Pathogenic Escherichia coli |
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| WANG Wen-cheng,ZHANG Gui-gang,WEI Guang-sen,LI Shang-bo,XU Chong-bo. Cloning of Heat-labile Enterotoxin Gene of Chicken Pathogenic Escherichia coli[J]. Chinese Journal of Veterinary Drug, 2002, 36(6): 26-28 |
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| Authors: | WANG Wen-cheng ZHANG Gui-gang WEI Guang-sen LI Shang-bo XU Chong-bo |
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| Affiliation: | WANG Wen-cheng1,ZHANG Gui-gang1,WEI Guang-sen1,LI Shang-bo1,XU Chong-bo2 |
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| Abstract: | Pathogenic avian Escherichia coli O78 is able to produce heat-labile enterotoxin (LT) with rabbit ileumligation test (RILT). Heat-labile enterotoxin gene was amplified from Pathogenic avian Escherichia coli O78 bypolymerase chain reaction (PCR). PCR products were isolated by 1% agarose gel electrophoresis and recovered byPCR preps DNA purification system. Then the heat-labile enterotoxin gene was inserted in pGEM-T vector. Bytransformation of JM109 and screening with Amp/IPTG/X-gal agar plate, we got recombinant strainJM109(pXCLT1). The recombinant plasmid pXCLT1 was studied in detail by restriction endonuclease analysis.Results showed that the recombinant plasmid carried heat-labile enterotoxin gene. |
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| Keywords: | Escherichia coli enterotxoin heat-labile enterotoxin gene gene cloning |
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