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| 高盐胁迫下的草原龙胆基因表达分析 | |
| 引用本文： | 王继刚,张坤,李葵花,冯国军,李玉花.高盐胁迫下的草原龙胆基因表达分析[J].北方园艺,2008(11). |
| 作者姓名： | 王继刚 张坤 李葵花 冯国军 李玉花 |
| 作者单位： | 1. 东北林业大学,生命科学学院,黑龙江,哈尔滨,150040 2. 西南林学院,学报编辑部,云南,昆明,650224 3. 哈尔滨市农业科学院,黑龙江,哈尔滨,150070 |
| 基金项目： | 引进国际先进农业科学技术计划(948计划) ， 黑龙江省公关课题 |
| 摘 要： | 为了更好的理解植物对盐胁迫的反应的分子机理,结合差减抑制杂交技术和基因芯片的方法,研究了草原龙胆对于高盐胁迫响应重要性和新基因.先利用草原龙胆50#叶片进行盐处理和非盐处理,并以此构建抑制差减文库.从此文库中选取部分克隆构建了草原龙胆基因芯片,进行了芯片分析,研究了高盐处理对非处理条件下的基因表达变化.在3 h的高盐胁迫下,基因芯片分析显示有36个单一基因有差异表达,大多数基因未见报道.这些基因的存在表明盐胁迫导致了草原龙胆复杂的胁迫响应. |
| 关 键 词： | 草原龙胆 芯片 抑制差减杂交 盐胁迫 |
Gene Expression of Eustoma Grandflorum under High Salinity Stress |
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| WANG Ji-gang,ZANG Kun,Li Kui-hua,FENG Guo-jun,LI Yu-hua.Gene Expression of Eustoma Grandflorum under High Salinity Stress[J].Northern Horticulture,2008(11). | |
| Authors: | WANG Ji-gang ZANG Kun Li Kui-hua FENG Guo-jun LI Yu-hua |
| Institution: | WANG Ji-gang1,ZANG Kun2,Li Kui-hua1,FENG Guo-jun3,LI Yu-hua1 (1.College of Life Science,Northeast Forestry University,Harbin,Heilongjiang 150040,China,2.Editorial Department of Academic Journal,Southwest Forestry University,Kunming,Yunnan 650224,3.Harbin Academy of Agricultural Sciences,Heilongjiang 150070,China) |
| Abstract: | To understand the molecular basis of plant responses to salt stress better,suppression subtractive hybridization(SSH) and microarray approaches were combined to identify the potential important or novel genes of Eustoma grandiflorum involved responses to severe salt stress.First,SSH libraries were constructed for the leave tissue of cultivated Eustoma grandiflorum 50# to compare salt treatment and non-treatment plants.Then a subset of clones from these SSH libraries were used to construct a Eustoma grandifl... |
| Keywords: | Eustoma Grandflorum Microarray Suppression subtractive hybridization Salt stress |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |