| 茶多酚固定化酶体外氧化产物茶黄素组成及其化学发光分析 |
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| 引用本文： | 丁兆堂,王秀峰,于海宁,沈生荣.茶多酚固定化酶体外氧化产物茶黄素组成及其化学发光分析[J].茶叶科学,2005,25(1):49-55. |
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| 作者姓名： | 丁兆堂 王秀峰 于海宁 沈生荣 |
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| 作者单位： | 山东农业大学园艺学院,山东,泰安,271018;浙江大学生命科学学院,浙江,杭州,310029;浙江大学茶叶研究所,浙江,杭州,310029 |
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| 基金项目： | 山东省优秀中青年科学家科研奖励基金 |
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| 摘 要： | 以红茶中提取的茶黄素粗提物和氧化前的茶多酚为对照,利用HPLC技术分析了纳米CaCO3固定化多酚氧化酶氧化产物的茶黄素组成;利用化学发光法进行了抗氧化活性研究。结果发现,固定化酶体外氧化产物中茶黄素组成以TF2A为主,茶黄素粗提物中四种茶黄素单体均占一定的比例;利用CuSO4-Vc-H2O2-酵母体系和Fe2+-H2O2-酵母体系进行化学发光研究,发现固定化酶体外氧化产物抗氧化活性较茶黄素粗提物和茶多酚强。
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| 关 键 词： | 纳米材料 固定化酶 茶黄素 化学发光 |
| 文章编号： | 1000-369X(2005)01-049-07 |
| 修稿时间： | 2004年12月6日 |
The Composition and Chemiluminescence Analysis of Theaflavins in Enzymatic Oxidation Products of Tea Polyphenols |
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| DING Zhao-tang,WANG Xiu-feng,YU Hai-ning,SHEN Sheng-rong.The Composition and Chemiluminescence Analysis of Theaflavins in Enzymatic Oxidation Products of Tea Polyphenols[J].Journal of Tea Science,2005,25(1):49-55. |
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| Authors: | DING Zhao-tang WANG Xiu-feng YU Hai-ning SHEN Sheng-rong |
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| Abstract: | In this paper, theaflavins extracts from black tea and tea polyphenols were used as control, composition of theaflavins in enzymatic oxidation products was analyzed by HPLC, and the antioxidant activity was investigated by the technique of chemiluminescence. Results showed that, TF2A was the main composition in enzymatic oxidation products. In theaflavins extracts from black tea, there mainly existed four kinds of theaflavins and their contents were not greatly fluctuated. By employing CuSO4-Vc-H2O2-yeast and Fe2 -H2O2-yeast systems, antioxidant activity of enzymatic oxidation products was better than theaflavins extracts from black tea and tea polyphenols. |
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| Keywords: | Nanopartical material Immobilized enzyme Teaflavins Chemiluminescence |
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