Inhibitory activities of the edible brown alga <Emphasis Type="Italic">Laminaria japonica</Emphasis> on glucose-mediated protein damage and rat lens aldose reductase |
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Authors: | You Kyung Son Seong Eun Jin Hyeung-Rak Kim Hee Chul Woo Hyun Ah Jung Jae Sue Choi |
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Institution: | (1) Division of Food Science and Biotechnology, Pukyong National University, Busan, 608-737, Korea;(2) Division of Applied Chemical Engineering, Pukyong National University, Busan, 608-739, Korea;(3) Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju, 561-756, Korea |
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Abstract: | Increased aldose reductase (AR)-related polyol pathway activity and the subsequent formation of advanced glycation end-products
(AGEs) have been implicated in the onset of diabetic complications. We have evaluated the inhibitory effects of the methanolic
extract and its fractions of Laminaria japonica on rat lens AR (RLAR) and AGE formation. The CH2Cl2 fraction was separated by high-performance liquid chromatography to yield active porphyrin derivatives, including pheophorbide
a (1) and pheophytin a (2), which were assayed for their anti-diabetic complications and yield. Compound 1 exhibited potent inhibitory activities against both AGE formation and RLAR, with respective half-maximal inhibitory concentration
(IC50) values of 49.4 and 12.3 μM. Conversely, compound 2 was found to be active against AGE formation, with an IC50 of 228.7 μM. For further elucidation of the structure–inhibitory activity relationship of the porphyrin derivatives, the
inhibitory activities of four commercially available porphyrin derivatives on AGE formation and RLAR were measured. The presence
of a carboxyl group and the absence of a phytyl group at the C-172 position of the porphyrin were found to contribute to the inhibitory effects towards both AGE formation and RLAR. These results
suggest that the L. japonica and its porphyrin derivatives may represent a potential functional food resource for further prevention of diabetic complications. |
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